Critical evaluation and further development of methods for testing ecotoxicity at multiple pH using Daphnia magna and Pseudokirchneriella subcapitata

Environmental Toxicology and Chemistry
Cecilie RendalKresten Ole Kusk

Abstract

To meet the requirements of risk assessment legislature regarding the ecotoxicity of ionizing compounds, the present study attempts to establish easy, robust methods for testing ecotoxicity at various pH levels. An overview is given of the buffering methods found in the literature. This is supplemented by a series of experiments where toxicity and ability to stabilize pH of seven common buffering compounds was tested on Daphnia magna and Pseudokirchneriella subcapitata. We consider a buffer applicable at a given concentration if the pH drift is below 0.2 pH units, and if there are no toxic effects. Twenty-four- and 48-h acute toxicity tests with D. magna were carried on a series of organic buffers with pH monitoring. Based on the experimental results it is possible to give recommendations for buffer concentrations for use in toxicity testing with D. magna at pH levels in the range of pH 6.0-7.8 for 48 h exposure, and pH 6.0-9.5 for 24 h exposure. Forty-eight- and 72-h growth inhibition tests with P. subcapitata were carried out, and recommendations for buffer concentrations at pH 7.5 and 8.0 are made for both 48 and 72 h of exposure.

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Aug 9, 2011·Environmental Toxicology and Chemistry·Cecilie RendalStefan Trapp

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Citations

Nov 9, 2012·Archives of Environmental Contamination and Toxicology·Aleicia HollandSusan H W Kinnear
Feb 16, 2013·Chemosphere·Gitte Gotholdt AnskjærKresten Ole Kusk
Sep 18, 2013·Environmental Science and Pollution Research International·Jonas BaumannJuliane Filser
Jul 30, 2014·Chemosphere·Gracia MárquezPedro M Álvarez
Oct 2, 2015·Bulletin of Environmental Contamination and Toxicology·Mirco BundschuhRalf Schulz
Aug 8, 2014·Environmental Toxicology and Chemistry·Jayne RobertsRoger van Egmond
Dec 8, 2015·Environmental Toxicology and Chemistry·Ricki R RosenfeldtMirco Bundschuh
Oct 1, 2019·Environmental Toxicology and Chemistry·Beate I EscherThomas F Parkerton
May 16, 2019·Environmental Geochemistry and Health·Hee-Jun KimHyun-Woo Kim

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