Critical features of a conserved RNA stem-loop important for feedback regulation of RNase E synthesis.

The Journal of Biological Chemistry
Alexis A Diwa, Joel G Belasco

Abstract

RNase E is an important regulatory enzyme that governs the principal pathway for mRNA degradation in Escherichia coli. This endonuclease controls its own synthesis via a feedback mechanism in which the longevity of rne (RNase E) mRNA is modulated by a cis-acting sensory element that responds to changes in cellular RNase E activity. Previous research has shown that this element is an RNA stem-loop (hp2) within the 5'-untranslated region of the rne transcript. Here we report studies involving mutational analysis and phylogenetic comparison that have identified the features of rne hp2 important for its function. These comprise an internal loop flanked on one side by a 2-bp stem and a hairpin loop and on the other side by a longer stem whose sequence is inconsequential. A search of bacterial genome sequences suggests that regulation by an hp2-like element may be a unique evolutionary adaptation of the rne transcript that is not shared by other mRNAs.

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Citations

Nov 8, 2002·Molecular Microbiology·Alexis A DiwaJoel G Belasco
Dec 14, 2006·Microbial Cell Factories·Christian Kemmer, Peter Neubauer
Jul 9, 2004·Journal of Molecular Biology·Anastasia J CallaghanBen F Luisi
Dec 1, 2015·PloS One·Ezekiel CrenshawAleister J Saunders
Aug 6, 2003·Journal of Molecular Biology·Claudio O GualerziCynthia L Pon
Jan 20, 2009·Journal of Molecular Biology·Stefan NordP Mikael Wikström
Oct 6, 2020·Frontiers in Microbiology·Diego A Vargas-Blanco, Scarlet S Shell
Apr 1, 2007·EcoSal Plus·Sidney R Kushner

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