Critical issues in chiral drug analysis in biological fluids by high-performance liquid chromatography

Journal of Chromatography. B, Biomedical Applications
J DucharmeR Farinotti

Abstract

This review article focuses on the specificities of chiral liquid chromatography, with particular emphasis on stability, stereoconversion, enantiomeric separation, recovery and drug concentration determinations. In addition, the paper presents an overview of the different steps which have to be followed for a chiral method to be validated. Sensitivity, selectivity, linearity, precision and accuracy all have to be ensured for three chemical entities, the two enantiomers and the racemate. Only accurate and precise concentrations of the parent drug and its metabolites will lead to the reliable description of their in vitro stability and in vivo body disposition.

References

Jan 1, 1990·Acta Pharmaceutica Nordica·G T Tucker
Aug 1, 1995·Journal of Pharmaceutical Sciences·S D RoyA Divor
May 1, 1993·Journal of Pharmaceutical Sciences·J ShimadaH Yamada
Sep 1, 1995·Journal of Pharmaceutical Sciences·M BrandlD Johnson

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Citations

Feb 9, 2002·Biopharmaceutics & Drug Disposition·G Gübitz, M G Schmid
Mar 21, 2007·Journal of Pharmaceutical and Biomedical Analysis·Igor Rafael dos Santos Magalhães, Pierina Sueli Bonato
Mar 17, 2004·British Journal of Pharmacology·Sylvie Barraud de LagerieFrançois Gimenez
Jun 29, 1999·Analytical Chemistry·D J Anderson

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