Critical Role for Molecular Iron in Coxiella burnetii Replication and Viability.

MSphere
Savannah E Sanchez, Anders Omsland

Abstract

Coxiella burnetii, the causative agent of Query (Q) fever in humans, is a highly infectious obligate intracellular bacterium. Following uptake into a host cell, C. burnetii replicates within a phagolysosome-derived compartment referred to as the Coxiella-containing vacuole (CCV). During infection, C. burnetii exhibits tropism for tissues related to iron storage and recycling (e.g., the liver and splenic red pulp), suggesting that pathogen physiology is linked to host iron metabolism. Iron has been described to have a limited role in C. burnetii virulence regulation, despite evidence that C. burnetii -infected host cells increase expression of transferrin receptors, thereby suggesting that active iron acquisition by the bacterium occurs upon infection. Through the use of host cell-free culture, C. burnetii was separated from the host cell in order to directly assess the role of different forms of iron in C. burnetii replication and viability, and therefore virulence. Results indicate that C. burnetii tolerates molecular iron over a broad concentration range (i.e., ∼0.001 to 1 mM) and undergoes gross loss of viability upon iron starvation. C. burnetii protein synthesis and energy metabolism, however, occur nearly uninhibited unde...Continue Reading

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Citations

Apr 30, 2021·Frontiers in Veterinary Science·Qingqing GaoSong Gao
Jun 3, 2021·Proceedings of the National Academy of Sciences of the United States of America·Qi YanYasuko Rikihisa
Jul 15, 2021·Pathogens and Disease·Savannah E Sanchez, Anders Omsland
May 20, 2021·Genome Biology and Evolution·Amanda E BrennerRahul Raghavan

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Methods Mentioned

BETA
RSA
fluorescence microscopy
scraping
PCR

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