Critical Role of Alternative M2 Skewing in miR-155 Deletion-Mediated Protection of Colitis

Frontiers in Immunology
Jintao LiYuzhang Wu

Abstract

Inflammatory bowel disease (IBD) is associated with dysregulation of both innate and adaptive immune response in the intestine. MicroRNA (miR)-155 is frequently expressed and functions in many immune cell types. Besides its function in adaptive immunity, miR-155 is a key regulator of the innate immune response in macrophages, dendritic cells, and even in epithelia cells. Although the roles of miR-155 within T and B lymphocytes in colitis have been reported, its function in innate immune cells has not been thoroughly examined. In this study, the dextran sulfate sodium (DSS)-induced colitis model was established in wild-type (WT) and miR-155-/- mice. Our results showed that miR-155 deficiency in macrophages recapitulated the alleviated colitis feature of miR-155-/- mice and appeared to skew toward the alterative M2 phenotype. Notably, the predominance of M2 in colon can result in dampened intestinal immune cell proliferation and inhibit CD4 T cell polarization toward Th1 and Th17. Moreover, C/EBPβ and SOCS1 were demonstrated as two key functional targets in this process. We also provided evidence for use of miR-155 inhibitor to treat colitis. Collectively, the findings highlight the central role of alternative M2 skewing for miR-...Continue Reading

References

Mar 21, 2007·The Journal of Immunology : Official Journal of the American Association of Immunologists·Philip SmithPadraic G Fallon
Apr 28, 2007·Science·Antony RodriguezAllan Bradley
Feb 6, 2009·Proceedings of the National Academy of Sciences of the United States of America·Maurizio CeppiPhilippe Pierre
Jan 5, 2010·Gastroenterology·Meaghan M HunterDerek M McKay
May 4, 2010·The Journal of Biological Chemistry·Claire E McCoyLuke A J O'Neill
Jul 14, 2010·Journal of Gastroenterology and Hepatology·Tomohisa TakagiToshikazu Yoshikawa
Jun 17, 2011·Nature·Kevin J Maloy, Fiona Powrie
Jun 21, 2011·Journal of Leukocyte Biology·Shelley B WeisserLaura M Sly
Jun 1, 2012·Proceedings of the National Academy of Sciences of the United States of America·Alicia ArranzChristos Tsatsanis
Oct 11, 2012·Nature Communications·Namiko HoshiRuslan Medzhitov
Apr 5, 2013·Immunological Reviews·Elena VigoritoRebecca Leyland
Sep 18, 2013·The Journal of Experimental Medicine·Heidi A SchreiberMichel C Nussenzweig
Jan 7, 2014·Proceedings of the National Academy of Sciences of the United States of America·Pamela V ChangRuslan Medzhitov
Sep 19, 2014·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Adrian T BilleterHiram C Polk
Apr 2, 2015·Immunology·Ryuichi Mashima
Dec 3, 2015·Nature Reviews. Gastroenterology & Hepatology·Heitor S P de Souza, Claudio Fiocchi
Aug 3, 2016·The Journal of Experimental Medicine·Wen-Hsien LiuChangchun Xiao
May 4, 2017·Biochemical and Biophysical Research Communications·Meng XuYujin Liu
Sep 16, 2017·Frontiers in Immunology·Jezrom Bokcaerin Self-FordhamSalvador Nares

❮ Previous
Next ❯

Citations

Sep 6, 2019·The Journal of Immunology : Official Journal of the American Association of Immunologists·Audrey PaolettiXavier Mariette
Oct 2, 2019·Frontiers in Immunology·Xiaoling Niu, Grant S Schulert
Dec 18, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Austin BoucherRichard Dahl
May 30, 2020·Progress in Neuro-psychopharmacology & Biological Psychiatry·G Anderson, M Maes
Mar 20, 2021·Frontiers in Cell and Developmental Biology·Xuebing HanGang Liu
May 11, 2021·Frontiers in Immunology·Junxia HuangJi Yang
Jul 27, 2021·Frontiers in Pharmacology·Jing ZhouSimin Chen

❮ Previous
Next ❯

Methods Mentioned

BETA
IECs
density gradient centrifugation
transfection
ELISA
PCR
Assay
flow cytometry
IEC

Software Mentioned

GraphPad
Prism

Related Concepts

Related Feeds