Critical role of dispensable genes in Mycoplasma agalactiae interaction with mammalian cells.

Infection and Immunity
Eric BaranowskiChristine Citti

Abstract

Mycoplasmas are minimal bacteria whose genomes barely exceed the smallest amount of information required to sustain autonomous life. Despite this apparent simplicity, several mycoplasmas are successful pathogens of humans and animals, in which they establish intimate interactions with epithelial cells at mucosal surfaces. To identify biological functions mediating mycoplasma interactions with mammalian cells, we produced a library of transposon knockout mutants in the ruminant pathogen Mycoplasma agalactiae and used this library to identify mutants displaying a growth-deficient pheonotype in cell culture. M. agalactiae mutants displaying a 3-fold reduction in CFU titers to nearly complete extinction in coculture with HeLa cells were identified. Mapping of transposon insertion sites revealed 18 genomic regions putatively involved in the interaction of M. agalactiae with HeLa cells. Several of these regions encode proteins with features of membrane lipoproteins and/or were involved in horizontal gene transfer with phylogenetically distant pathogenic mycoplasmas of ruminants. Two mutants with the most extreme phenotype carry a transposon in a genomic region designated the NIF locus which encodes homologues of SufS and SufU, two pr...Continue Reading

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Citations

Jul 23, 2013·Nucleic Acids Research·Olga TsoyArcady Mushegian
Jun 5, 2014·PloS One·Shukriti SharmaGlenn F Browning
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Nov 27, 2014·MBio·Emilie Dordet-FrisoniChristine Citti
Nov 12, 2019·Frontiers in Microbiology·Emilie Dordet-FrisoniChristine Citti
Dec 19, 2019·Frontiers in Microbiology·Xifang ZhuEric Baranowski

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