Critical study of common conditions of storage of glucocorticoids and catecholamines in 24-h urine collected during resting and exercising conditions

Clinica Chimica Acta; International Journal of Clinical Chemistry
C GouarneM Duclos

Abstract

Except immediate freezing of the samples, no practical method has been validated for preservation of glucocorticoids and catecholamines in 24-h urine collection. Furthermore, the influence of urine storage at bladder temperature during periods of different lengths and the effect of prior exercise on preservation of these hormones in the bladder have not been investigated until now. Ten healthy volunteers collected their urine both after a resting and after an exercise session. Urine was aliquoted into tubes which were stored during 24 h in the presence or in the absence of preservatives and at different temperatures. Two samples were stored either 3 or 9 h at 37 degrees C (bladder temperature) without additive. When collecting 24-h urine samples for glucocorticoids determination, sample can be stored at room temperature during the 24-h collection period without compromising glucocorticoids preservation. When collecting 24-h urine samples for catecholamines determination, samples have to be chilled without preservative during the whole of the collection period. If the samples have to be stored at room temperature, HCl should be used. Moreover, we report for the first time that catecholamines can be degraded in the bladder and th...Continue Reading

References

Dec 1, 1976·Acta Physiologica Scandinavica·B Nielsen, C T Davies
Dec 16, 1997·Clinical Journal of Sport Medicine : Official Journal of the Canadian Academy of Sport Medicine·L HorneA Janowska-Wieczorek
Nov 16, 2002·Journal of Applied Physiology·M DuclosD Bonnemaison

❮ Previous
Next ❯

Citations

Mar 24, 2012·Animal : an International Journal of Animal Bioscience·C MapiyeV Muchenje
Jan 29, 2013·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Bardia JamaliFarzad Kobarfard
Nov 26, 2009·Clinical Chemistry and Laboratory Medicine : CCLM·Norman B RobertsMansour Sargazi
Jun 4, 2013·Applied Physiology, Nutrition, and Metabolism = Physiologie Appliquée, Nutrition Et Métabolisme·Julien AucouturierPascale Duché
Sep 1, 2015·Psychoneuroendocrinology·Sonja L van OckenburgKarin A M Janssens
May 16, 2014·Clinical Chemistry and Laboratory Medicine : CCLM·Ming LiAiling Song

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bladder Carcinoma In Situ

Bladder Carcinoma In Situ is a superficial bladder cancer that occurs on the surface layer of the bladder. Discover the latest research on this precancerous condition in this feed.