Critical threonine and aspartic acid residues within the I domains of beta 2 integrins for interactions with intercellular adhesion molecule 1 (ICAM-1) and C3bi.

The Journal of Biological Chemistry
T KamataY Takada

Abstract

Integrins mediate signal transduction through interactions with multiple cellular or extracellular matrix ligands. Evidence is accumulating that the I (or A) domain, a approximately 200-residue inserted sequence in some integrin alpha subunits, mediates ligand binding. We have previously shown that Thr-221 of the putative ligand binding sites within alpha 2 I domain of alpha 2 beta 1 is critical for binding to collagen (Kamata, T., and Takada, Y. (1994) J. Biol. Chem. 269, 26006-26010). Here we report that the mutation of Thr-206 of alpha L blocks intercellular adhesion molecule 1 (ICAM-1) binding to alpha L beta 2 and mutation of Thr-209 of alpha M blocks ICAM-1 and C3bi binding to alpha M beta 2. The data indicate the Thr residues of alpha M and alpha L corresponding to Thr-221 of alpha 2 are critically involved in the ligand interaction with beta 2 integrins. The mutations of the Asp-137 and Asp-239 of alpha L also block ICAM-1 binding to alpha L beta 2, as do the corresponding Asp residues of alpha 2 or alpha M in collagen/alpha 2 beta 1 or C3bi/alpha M beta 2 interactions, respectively. These data suggest that these Thr and Asp residues, conserved among I domains, are critical for interaction with structurally distinct lig...Continue Reading

References

Jan 1, 1992·Analytical Biochemistry·W P Deng, J A Nickoloff
Aug 2, 1990·Nature·T A Springer
Sep 14, 1990·Biochemical and Biophysical Research Communications·A L BackD D Hickstein
Jan 1, 1991·The Journal of Clinical Investigation·E Ruoslahti
Mar 1, 1991·The Journal of Cell Biology·C A PraterW A Frazier
Aug 1, 1990·The Journal of Cell Biology·M J IgnatiusL F Reichardt
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·W S ArgravesP F Goetinck
Sep 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·S D WrightS C Silverstein
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·F Sanchez-MadridT A Springer
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·T UedaM A Arnaout
Mar 1, 1993·The Journal of Cell Biology·R C LandisN Hogg

❮ Previous
Next ❯

Citations

Jun 15, 1996·Journal of Cellular Biochemistry·M Stewart, N Hogg
Jan 1, 1996·International Journal of Clinical & Laboratory Research·G BertonC A Lowell
Jul 17, 1999·Immunopharmacology·G D RossJ Vetvicková
May 14, 1999·Immunology Today·M E Binnerts, Y van Kooyk
Dec 24, 1997·Matrix Biology : Journal of the International Society for Matrix Biology·Y TakadaX P Zhang
Oct 1, 1996·Current Opinion in Cell Biology·E Y Jones
Oct 1, 1996·Current Opinion in Cell Biology·M J Humphries
May 3, 2003·Current Opinion in Structural Biology·Martin J HumphriesA Paul Mould
Apr 18, 2008·The Journal of Biological Chemistry·Lester J LambertFrancesca M Marassi
Apr 15, 1997·European Journal of Biochemistry·C G GahmbergP Kotovuori
Jan 1, 1997·Annual Review of Biochemistry·C Chothia, E Y Jones
May 15, 1997·The Journal of Clinical Investigation·J C Loftus, R C Liddington
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·C LuT A Springer
May 11, 2000·Proceedings of the National Academy of Sciences of the United States of America·J R HuthD E Staunton
May 4, 2002·Annual Review of Biophysics and Biomolecular Structure·Motomu ShimaokaTimothy A Springer
Sep 24, 1999·Journal of Molecular Biology·J KallenU Hommel
Aug 1, 2006·Molecular Immunology·Bert J C Janssen, Piet Gros
Aug 28, 2007·Chemical Biology & Drug Design·Helena Yusuf-MakagiansarTeruna J Siahaan
May 10, 2008·The Journal of Comparative Neurology·Jun-Ping CaoDian-Shuai Gao
Nov 14, 1997·The Journal of Biological Chemistry·J EmsleyR C Liddington
Jun 6, 2000·The Journal of Biological Chemistry·J M HigginsM B Brenner
Sep 6, 1996·The Journal of Biological Chemistry·E C TozerJ C Loftus
Dec 11, 2002·The Journal of Biological Chemistry·Stephen BallAdrian Shuttleworth

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.

Alternative Complement Pathway

The Alternative Complement Pathway is part of the innate immune system, and activation generates membrane attack complexes that kill pathogenic cells. Discover the latest research on the Alternative Complement Pathway.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.