Crk adaptor proteins act as key signaling integrators for breast tumorigenesis.

Breast Cancer Research : BCR
Kelly E FathersMorag Park

Abstract

CT10 regulator of kinase (Crk) adaptor proteins (CrkI, CrkII and CrkL) play a role in integrating signals for migration and invasion of highly malignant breast cancer cell lines. This has important implications, as elevated CrkI/II protein levels were observed in a small cohort of breast cancer patients, which identified a potential role for Crk proteins in breast cancer progression. Numerous in vitro studies identified a role for Crk proteins in cell motility, but little is known about how Crk proteins contribute to breast cancer progression in vivo. The clinical significance of Crk proteins in human breast cancer was assessed by analyzing published breast cancer datasets using a gene expression signature that was generated following CrkII over-expression and by examining Crk protein expression in tissue microarrays of breast tumors (n = 254). Stable knockdown of Crk (CrkI/CrkII/CrkL) proteins was accomplished using a short hairpin RNA (shRNA)-mediated approach in two basal breast cancer cell lines, MDA-231 1833TR and SUM1315, where the former have a high affinity to form bone metastases. Both in vitro assays (cell migration, invasion, soft agar growth) and in vivo experiments (intra-cardiac, tibial and mammary fat pad injecti...Continue Reading

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Citations

Dec 12, 2012·Genes & Cancer·Emily S Bell, Morag Park
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Methods Mentioned

BETA
X-ray
surgical resection
GTPases
pull-down
pulldown
nucleotide exchange
biopsies
electrophoresis

Software Mentioned

ImageScope
ImageJ
Scion Image
Odyssey
Infinity Analyze

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