CrMAPK3 regulates the expression of iron-deficiency-responsive genes in Chlamydomonas reinhardtii

BMC Biochemistry
Xiaowen FeiXiaodong Deng

Abstract

Under iron-deficient conditions, Chlamydomonas exhibits high affinity for iron absorption. Nevertheless, the response, transmission, and regulation of downstream gene expression in algae cells have not to be investigated. Considering that the MAPK pathway is essential for abiotic stress responses, we determined whether this pathway is involved in iron deficiency signal transduction in Chlamydomonas. Arabidopsis MAPK gene sequences were used as entry data to search for homologous genes in Chlamydomonas reinhardtii genome database to investigate the functions of mitogen-activated protein kinase (MAPK) gene family in C. reinhardtii under iron-free conditions. Results revealed 16 C. reinhardtii MAPK genes labeled CrMAPK2-CrMAPK17 with TXY conserved domains and low homology to MAPK in yeast, Arabidopsis, and humans. The expression levels of these genes were then analyzed through qRT-PCR and exposure to high salt (150 mM NaCl), low nitrogen, or iron-free conditions. The expression levels of these genes were also subjected to adverse stress conditions. The mRNA levels of CrMAPK2, CrMAPK3, CrMAPK4, CrMAPK5, CrMAPK6, CrMAPK8, CrMAPK9, and CrMAPK11 were remarkably upregulated under iron-deficient stress. The increase in CrMAPK3 expressio...Continue Reading

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Citations

May 15, 2020·International Journal of Molecular Sciences·Gretchen E Kroh, Marinus Pilon
May 16, 2020·International Journal of Molecular Sciences·Aitor Gomez-OsunaAngel Llamas

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Methods Mentioned

BETA
environmental stress
PCR
transgenic

Software Mentioned

BLAST
MEME
BLASTP
mPLoc2
MEGA6
PROSITE
iCycler
ExPASy
GSDS
SMART

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