Crocetin inhibits leukocyte adherence to vascular endothelial cells induced by AGEs.

Journal of Ethnopharmacology
Min XiangWen-Na Li

Abstract

Advance glycation end products (AGEs) have been postulated to play an important role in diabetic complications such as atherosclerosis disease. Adhesion and migration of leukocyte to endothelial cells (EC) is one of the early key steps in the pathogenesis. Crocetin is an important ingredient of diet in India and also used in various systems of indigenous medicine. In this study, we investigated effect of crocetin on leukocyte adherence to bovine endothelial cells (BEC) induced by AGEs in vitro and the possible mechanisms involved. BEC were pre-incubated with crocetin (0.01, 0.1, and 1 microM) for 12 h and exposed to AGEs (100 microg/ml). Cells proliferation was determined by MTT; leukocyte-endothelial cell adhesion was assayed by myeloperoxidase methods; intercellular adhesion molecular-1 (ICAM-1) protein expression was studied by immunocytochemistry and mitochondrial membrane potential (MMP) was analyzed by the retention of rhodamine 123 (RH123); furthermore, levels of anion (O(2)(-)), malonicdialdehyde (MDA) in super cells culture and superoxide dismutase (SOD) in cells were also detected, respectively. Results demonstrated that crocetin could inhibit AGE-induced BEC growth suppression and significantly reduce adhesion rate o...Continue Reading

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Citations

Sep 11, 2010·Critical Reviews in Food Science and Nutrition·S Zahra Bathaie, S Zeinab Mousavi
Feb 27, 2013·Journal of Medicinal Food·Ziba RajaeiSomayeh Shafiee
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Feb 6, 2020·Archives of Physiology and Biochemistry·Babak RoshanravanTahereh Farkhondeh

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