Crocodilian perivitelline membrane-bound sperm detection

Zoo Biology
Lauren Augustine

Abstract

Advanced reproductive technologies (ART's) are often employed with various taxa to enhance captive breeding programs and maintain genetic diversity. Perivitelline membrane-bound (PVM-bound) sperm detection has previously been demonstrated in avian and chelonian species as a useful technique for breeding management. In the absence of embryotic development within an egg, this technique can detect the presence of sperm trapped on the oocyte membrane confirming breeding, male reproductive status, and pair compatibility. PVM-bound sperm were successfully detected in three clutches of Cuban crocodile (Crocodylus rhombifer) eggs at the Smithsonian's National Zoological Park (NZP) for the first time in any crocodilian species. PVM-bound sperm were detected in fresh and incubated C. rhombifer eggs, as well as eggs that were developing (banded) and those that were not (not banded). The results of this study showed significant differences in average sperm densities per egg between clutches (p = 0.001). Additionally, there was not a significant difference within clutches between eggs that banded and those that did not band (Clutch A, p = 0.505; Clutch B, p = 0.665; Clutch C, p = 0.266). The results of this study demonstrate the necessity t...Continue Reading

References

Feb 1, 1977·Biological Reviews of the Cambridge Philosophical Society·G C PackardJ J Roth
Oct 1, 1977·Biology of Reproduction·M R Bakst, B Howarth
Jul 1, 1997·Animal Reproduction Science·G J Wishart
Apr 11, 2006·The Quarterly Review of Biology·Hugh Drummond
Dec 19, 2013·PloS One·Matthew L BrienKeith A Christian
Jan 1, 2015·Conservation Physiology·Kaitlin E CroyleThomas Jensen

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