Crohn's disease patients have more IgG-binding fecal bacteria than controls.

Clinical and Vaccine Immunology : CVI
Hermie J M HarmsenGerard Dijkstra

Abstract

In Crohn's disease (CD), chronic gut inflammation leads to loss of mucosal barrier integrity. Subsequent leakage of IgG to the gut could produce an increase of IgG coating of intestinal bacteria. We investigated if there is more IgG coating in patients than in volunteers and whether this is dependent on the host IgG response or on the gut bacteria. Fecal and serum samples were obtained from 23 CD patients and 11 healthy volunteers. Both the in vivo IgG-coated fecal bacteria and in vitro IgG coating after serum addition were measured by flow cytometry and related to disease activity. The bacterial composition in feces was determined using fluorescence in situ hybridization. The IgG-binding capacities of Escherichia coli strains isolated from feces of patients and volunteers were assessed. The results showed that the in vivo IgG-coated fraction of fecal bacteria of patients was slightly larger than that of volunteers but significantly larger after incubation with either autologous or heterologous serum. This was dependent on the bacteria and independent of disease activity or the serum used. The presence of more Enterobacteriaceae and fewer faecalibacteria in patient feces was confirmed. E. coli isolates from patients bound more ...Continue Reading

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Citations

Jan 27, 2015·Cell Host & Microbe·Jason L KubinakJune L Round
Dec 17, 2015·Scientific Reports·Mehdi Sadaghian SadabadKlaas Nico Faber
Dec 5, 2015·Frontiers in Immunology·Ludovica F ButtóDirk Haller
Apr 14, 2015·Systematic and Applied Microbiology·Senthil Alias SankarPierre-Edouard Fournier
Jun 2, 2016·Critical Reviews in Food Science and Nutrition·Choon Yoong CheokGun Hean Chong
Aug 16, 2019·Mucosal Immunology·Delphine SterlinGuy Gorochov
Aug 6, 2020·Genes and Immunity·Katharina Kappler, Thierry Hennet
May 13, 2021·Neurology. Neuroimmunology and Neuroinflammation·Delphine SterlinGuy Gorochov
Jun 25, 2021·Frontiers in Immunology·Anna TorunBeata Pyrzynska

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