Cross-chest median nerve transfer: a new model for the evaluation of nerve regeneration across a 40 mm gap in the rat

Journal of Neuroscience Methods
N SinisMax Haerle

Abstract

A new animal model for the study of nerve regeneration in rats across a 40 mm gap between both median nerves is described. For autologous grafting, the ulnar nerves were dissected and sutured together. From the left median nerve, they were transplanted across the chest to the right median nerve. Animals having undergone this operation were observed for 12 months and periodically assessed using the grasping test and measurements of body-weight. For histological analysis rats were sacrificed after this period and axon counts were determined at the suture points of operated animals and in the median nerve of non-operated animals. Functional recovery could be seen, although partially, beginning as early as the fifth postoperative month, as demonstrated by the grasping test. Quantification of the number of axons demonstrated axonal regeneration across all three coaptation points. This model provides a new approach for analysis of long distance peripheral nerve regeneration without impairment of behaviour.

References

Feb 1, 1991·Journal of Neuroscience Methods·R E Sporel-OzakatW H Gispen
Jul 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·C R Worthington
Jul 19, 2001·Journal of Neuroscience Methods·A S VarejãoA M Cabrita
Jan 28, 2005·The European Journal of Neuroscience·Orlando Guntinas-LichiusDoychin N Angelov
Feb 9, 2005·Experimental Neurology·Grant A Robinson, Roger D Madison
Feb 11, 2005·Journal of the Peripheral Nervous System : JPNS·Eleana BontiotiLars B Dahlin
May 18, 2005·Biotechnology and Applied Biochemistry·Ruben Y KannanAlexander M Seifalian
Jun 23, 2005·Journal of Reconstructive Microsurgery·James M KernsMark Gonzalez

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Citations

Apr 11, 2008·Tissue Engineering. Part a·Amauri PierucciAlexandre Leite Rodrigues de Oliveira
May 30, 2009·Biomedical Microdevices·Valeria ChionoGianluca Ciardelli
Oct 19, 2010·Progress in Neurobiology·Ronald DeumensGary A Brook
Jun 10, 2009·Journal of Neuroscience Methods·Frank WerdinNektarios Sinis
Apr 15, 2011·Annals of Anatomy = Anatomischer Anzeiger : Official Organ of the Anatomische Gesellschaft·Nektarios SinisFrank Werdin
Jan 30, 2015·Journal of Neuroscience Methods·Stefano Geuna
Aug 9, 2016·American Journal of Transplantation : Official Journal of the American Society of Transplantation and the American Society of Transplant Surgeons·B KernG Brandacher
Jul 19, 2019·Frontiers in Cellular Neuroscience·Giulia RonchiGiovanna Gambarotta
Apr 17, 2010·Annals of Plastic Surgery·Epaminondas KostopoulosJulia K Terzis

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