Cross-competition in editing of chloroplast RNA transcripts in vitro implicates sharing of trans-factors between different C targets

The Journal of Biological Chemistry
Wade P HellerMaureen R Hanson

Abstract

C-->U plant organellar RNA editing is required for the translation of evolutionarily conserved and functional proteins. 28 different C targets of RNA editing have been identified in maize chloroplasts, and hundreds of Cs are edited in mitochondria. Mutant analysis in Arabidopsis has indicated that absence of a single site-specific recognition protein can result in loss of editing of a single C target, raising the possibility that each C target requires a recognition protein. Here we show that transcripts encompassing two editing sites, ZMrpoB C467 and ZMrps14 C80, can compete editing activity from each other in vitro despite limited sequence similarity. The signal causing competition overlaps a 5'-cis element required for editing efficiency. A single five-nucleotide mutation spanning the region from -20 to -16 relative to the edited C of rpoB C467 is sufficient to eliminate its substrate editing as well as its ability to compete editing activity from rps14 C80 substrates. A corresponding mutation in an rps14 C80 competitor likewise eliminated its ability to compete editing activity from rpoB C467 substrates. Taken together, our results indicate that the RNA sequences mediating both editing efficiency and cross-competition are h...Continue Reading

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Citations

Jul 4, 2008·The Journal of Biological Chemistry·Daniil VerbitskiyMizuki Takenaka
Oct 26, 2011·Biochemistry. Biokhimii︠a︡·B Castandet, A Araya
Aug 15, 2009·BMC Evolutionary Biology·Michael TillichChristian Schmitz-Linneweber
Mar 3, 2010·Annual Review of Plant Biology·David B SternMaureen R Hanson
Oct 13, 2010·Cellular and Molecular Life Sciences : CMLS·Volker Knoop
Dec 1, 2009·IUBMB Life·Michael W Gray
Oct 20, 2009·Gene Regulation and Systems Biology·Eva M del Campo
May 28, 2020·Genetics and Molecular Biology·Nureyev F RodriguesRogerio Margis

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