Cross-evaluation of five slidemakers and three automated image analysis systems: The pitfalls of automation?

International Journal of Laboratory Hematology
Imane Saad AlbichrGautier Detry

Abstract

Automated slidemakers and stainers and digital microscopes are coupled with haematology analysers to achieve better efficiency and cost-effectiveness. This study evaluates the integrated performance of slidemakers and digital microscopes commonly available on the market. We compared the percentage of neutrophils for five slidemakers (two Siemens Advia Autoslides, a SysmexSP-10 and SP-50 and an Abbot Alinity hs) and a Horiba Hemaprep to the corresponding haematology analyser data (Siemens Advia 2120i, Sysmex XN and Abbot Alinity hq). Differential leucocyte counting (DLC) was performed on three different CellaVision digital microscopes (DM96, DM1200 and DI-60) and manually. The quality of the smears was assessed using a CellaVision SmearChecker. We observed a significant positive absolute bias (P < .05) for the percentage of neutrophils with the Autoslide and Alinity hs smears on the digital microscopes, but not when DLC was performed manually. The SP-10 and SP-50 showed no bias regardless of the DLC method. No bias was observed for the Hemaprep smears. All the smears had an acceptable monolayer quality, stain intensity and colour. All smears, except those from Sp-10, were of an acceptable length. Users should be aware of a poten...Continue Reading

References

Jul 19, 2005·Laboratory Hematology : Official Publication of the International Society for Laboratory Hematology·P W BarnesUNKNOWN international consensus group for hematology
Jun 28, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Mikael RousselUNKNOWN International Council for Standardization in Hematology (ICSH)
Jul 17, 2013·Journal of Pathology Informatics·Lik Hang LeeChristopher Naugler
Sep 14, 2013·International Journal of Laboratory Hematology·W BrownB D Hedley
Jan 31, 2014·Revista brasileira de hematologia e hemoterapia·Eberson Damião Dos Santos de BitencourtTerezinha Munhoz
Feb 14, 2015·Clinics in Laboratory Medicine·Lydie Da Costa
Jul 5, 2017·Clinical Chemistry and Laboratory Medicine : CCLM·Hyeong Nyeon KimYeo-Min Yun
May 3, 2019·International Journal of Laboratory Hematology·Alexander KratzUNKNOWN International Council for Standardization in Haematology

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Citations

Jan 21, 2021·Clinical Chemistry and Laboratory Medicine : CCLM·Sumi YoonYeo-Min Yun

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