Cross-Feeding among Probiotic Bacterial Strains on Prebiotic Inulin Involves the Extracellular exo -Inulinase of Lactobacillus paracasei Strain W20

Applied and Environmental Microbiology
Markus C L BogerLubbert Dijkhuizen

Abstract

Probiotic gut bacteria employ specific metabolic pathways to degrade dietary carbohydrates beyond the capabilities of their human host. Here, we report how individual commercial probiotic strains degrade prebiotic (inulin type) fructans. First, a structural analysis of commercial fructose oligosaccharide-inulin samples was performed. These β-(2-1)-fructans differ in termination by either glucose (GF) or fructose (FF) residues, with a broad variation in the degrees of polymerization (DPs). The growth of individual probiotic bacteria on short-chain inulin (sc-inulin) (Frutafit CLR), a β-(2-1)-fructan (DP 2 to DP 40), was studied. Lactobacillus salivarius W57 and other bacteria grew relatively poorly on sc-inulin, with only fractions of DP 3 and DP 5 utilized, reflecting uptake via specific transport systems followed by intracellular metabolism. Lactobacillus paracasei subsp. paracasei W20 completely used all sc-inulin components, employing an extracellular exo-inulinase enzyme (glycoside hydrolase family GH32 [LpGH32], also found in other strains of this species); the purified enzyme converted high-DP compounds into fructose, sucrose, 1-kestose, and F2 (inulobiose). The cocultivation of L. salivarius W57 and L. paracasei W20 on s...Continue Reading

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Citations

Jan 10, 2020·MSphere·Zhi WangGabrielle Potocki-Veronese
Jun 11, 2020·Critical Reviews in Food Science and Nutrition·Jagrani MinjRakesh Kumar Sharma
Oct 4, 2020·Applied and Environmental Microbiology·Yuanting ZhuDavid A Mills
Nov 21, 2019·Journal of Agricultural and Food Chemistry·Markus BögerLubbert Dijkhuizen
Aug 2, 2021·Current Opinion in Biotechnology·Francesca TurroniDouwe van Sinderen
Aug 18, 2021·International Journal of Biological Macromolecules·Aldrine KiluaMichihiro Fukushima

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