Cross-feeding modulates the rate and mechanism of antibiotic resistance evolution in a model microbial community of Escherichia coli and Salmonella enterica.

PLoS Pathogens
Elizabeth M AdamowiczWilliam R Harcombe

Abstract

With antibiotic resistance rates on the rise, it is critical to understand how microbial species interactions influence the evolution of resistance. In obligate mutualisms, the survival of any one species (regardless of its intrinsic resistance) is contingent on the resistance of its cross-feeding partners. This sets the community antibiotic sensitivity at that of the 'weakest link' species. In this study, we tested the hypothesis that weakest link dynamics in an obligate cross-feeding relationship would limit the extent and mechanisms of antibiotic resistance evolution. We experimentally evolved an obligate co-culture and monoculture controls along gradients of two different antibiotics. We measured the rate at which each treatment increased antibiotic resistance, and sequenced terminal populations to question whether mutations differed between mono- and co-cultures. In both rifampicin and ampicillin treatments, we observed that resistance evolved more slowly in obligate co-cultures of E. coli and S. enterica than in monocultures. While we observed similar mechanisms of resistance arising under rifampicin selection, under ampicillin selection different resistance mechanisms arose in co-cultures and monocultures. In particular,...Continue Reading

References

Jul 20, 2007·Nature Reviews. Genetics·Adam Eyre-Walker, Peter D Keightley
Feb 17, 2009·The Journal of Antimicrobial Chemotherapy·Klas I UdekwuBruce R Levin
Dec 17, 2009·BMC Infectious Diseases·Itzhak Brook
Jan 27, 2010·Evolution; International Journal of Organic Evolution·William Harcombe
Jun 10, 2011·Molecular Microbiology·Steve P BernierJean-Marc Ghigo
May 23, 2012·PLoS Biology·Diane LawrenceTimothy G Barraclough
Sep 8, 2012·Current Opinion in Microbiology·Gautam Dantas, Morten O A Sommer
Feb 26, 2013·BMC Evolutionary Biology·Alejandra Rodríguez-VerdugoOlivier Tenaillon
May 11, 2013·American Journal of Epidemiology·Ian H SpicknallJoseph N S Eisenberg
Aug 7, 2013·BMC Evolutionary Biology·Daniel C Angst, Alex R Hall
Aug 7, 2013·Molecular Systems Biology·Eugene A YurtsevJeff Gore
Aug 13, 2013·PLoS Genetics·Norbert S HillPetra Anne Levin
Aug 16, 2013·Proceedings of the National Academy of Sciences of the United States of America·Nicole M VegaJames J Collins
May 20, 2014·Methods in Molecular Biology·Daniel E Deatherage, Jeffrey E Barrick
Jun 10, 2014·Evolution; International Journal of Organic Evolution·Glen D'SouzaChristian Kost
Jul 30, 2014·Frontiers in Microbiology·Erica C Seth, Michiko E Taga
Oct 8, 2014·Antimicrobial Agents and Chemotherapy·Usha StiefelMichelle M Nerandzic
Apr 16, 2016·Nature Reviews. Microbiology·Asher BraunerNathalie Q Balaban
May 20, 2016·Proceedings of the National Academy of Sciences of the United States of America·Eugene Anatoly YurtsevJeff Gore
Jun 18, 2016·PLoS Computational Biology·Sebastian GermerodtChristian Kost
Dec 7, 2016·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Teppo HiltunenAnna-Liisa Laine
Dec 28, 2016·PLoS Biology·Robin A SorgJan-Willem Veening
Mar 8, 2017·Molecular Systems Biology·Mattia ZampieriUwe Sauer
Apr 11, 2017·Nature Reviews. Microbiology·Terence S CroftsGautam Dantas
Aug 30, 2017·Journal of Bacteriology·Özhan ÖzkayaRoberto Balbontín
Oct 27, 2017·FEMS Microbiology Reviews·Johan Bengtsson-PalmeD G Joakim Larsson
Dec 21, 2017·MBio·Sónia CastanheiraFrancisco García-Del Portillo
Mar 15, 2018·PLoS Biology·Christoph Ratzke, Jeff Gore
Mar 23, 2018·The ISME Journal·Isabel FrostKevin R Foster
May 26, 2018·Natural Product Reports·Glen D'SouzaChristian Kost
Jun 22, 2018·PLoS Computational Biology·Sylvie Estrela, Sam P Brown
Jul 12, 2018·The ISME Journal·Elizabeth M AdamowiczWilliam R Harcombe

❮ Previous
Next ❯

Citations

Aug 23, 2020·International Journal of Molecular Sciences·Chris R TaittGary J Vora
Dec 29, 2020·Computational and Structural Biotechnology Journal·Johan Bengtsson-Palme
Feb 19, 2021·PLoS Computational Biology·Yaron Goldberg, Jonathan Friedman

❮ Previous
Next ❯

Methods Mentioned

BETA
Illumina sequencing
Hi-Seq

Software Mentioned

R
Stata
BreSeq

Related Concepts

Related Feeds

Allergy & Infectious Diseases (ASM)

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance (ASM)

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.

Allergy & Infectious Diseases

Allergies result from the hyperreactivity of the immune system to some environmental substance and can be life-threatening. Infectious diseases are caused by organisms including bacteria, viruses, fungi and parasites. They can be transmitted different ways, such as person-to-person. Here is the latest research on allergy and infectious diseases.

Antimicrobial Resistance

Antimicrobial resistance poses a significant threat to the continued successful use of antimicrobial agents for the treatment of bacterial infections.