Cross-genera SSR transferability in cacti revealed by a case study using Cereus (Cereeae, Cactaceae)

Genetics and Molecular Biology
Juliana Rodrigues BombonatoFernando Faria Franco

Abstract

The study of transferability of simple sequence repeats (SSR) among closely related species is a well-known strategy in population genetics, however transferability among distinct genera is less common. We tested cross-genera SSR amplification in the family Cactaceae using a total of 20 heterologous primers previously developed for the genera Ariocarpus, Echinocactus, Polaskia and Pilosocereus, in four taxa of the genus Cereus: C. fernambucensis subsp. fernambucensis, C. fernambucensis subsp. sericifer, C. jamacaru and C. insularis. Nine microsatellite loci were amplified in Cereus resulting in 35.2% of success in transferability, which is higher than the average rate of 10% reported in the literature for cross-genera transferability in eudicots. The genetic variation in the transferred markers was sufficient to perform standard clustering analysis, indicating each population as a cohesive genetic cluster. Overall, the amount of genetic variation found indicates that the transferred SSR markers might be useful in large-scale population studies within the genus Cereus.

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Citations

Dec 19, 2019·Molecular Biology Reports·Wellington Santos FavaGecele Matos Paggi
Sep 9, 2020·Journal of Ethnopharmacology·Éverton da Silva SantosRegina Aparecida Correia Gonçalves
Sep 2, 2021·Anais Da Academia Brasileira De Ciências·Angela Verónica GutiérrezFrancisco Pablo Ortega-Baes

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Methods Mentioned

BETA
PCR

Software Mentioned

Populations
Arlequin
Clumpp
Distruct
FreeNA
FSTAT
STRUCTURE
GenAlEx
Micro
Checker

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