Cross-genotype AR3-specific neutralizing antibodies confer long-term protection in injecting drug users after HCV clearance.

Journal of Hepatology
Sabrina J MeratJanke Schinkel

Abstract

In order to design an effective vaccine against hepatitis C virus (HCV) infection, it is necessary to understand immune protection. A number of broadly reactive neutralizing antibodies have been isolated from B cells of HCV-infected patients. However, it remains unclear whether B cells producing such antibodies contribute to HCV clearance and long-term immune protection against HCV. We analysed the B cell repertoire of 13 injecting drug users from the Amsterdam Cohort Study, who were followed up for a median of 17.5 years after primary infection. Individuals were classified into 2 groups based on the outcome of HCV infection: 5 who became chronically infected either after primary infection or after reinfection, and 8 who were HCV RNA negative following spontaneous clearance of ≥1 HCV infection(s). From each individual, 10,000 CD27+IgG+B cells, collected 0.75 year after HCV infection, were cultured to characterize the antibody repertoire. Using a multiplex flow cytometry-based assay to study the antibody binding to E1E2 from genotype 1 to 6, we found that a high frequency of cross-genotype antibodies was associated with spontaneous clearance of 1 or multiple infections (p = 0.03). Epitope specificity of these cross-genotype anti...Continue Reading

Citations

Feb 23, 2020·Vaccines·Joshua D DuncanJonathan K Ball
Nov 5, 2020·The Journal of General Virology·Mphatso D KalemeraJoe Grove
Apr 29, 2020·Cold Spring Harbor Perspectives in Medicine·Mansun Law
Nov 3, 2020·Current Opinion in Virology·Johnasha D StuartArash Grakoui
Apr 18, 2019·Journal of Hepatology·Florian WrenschThomas F Baumert
Jun 3, 2021·Viruses·Itai YechezkelNetanel Tzarum
Aug 11, 2021·Viruses·Christopher C PhelpsJonathan R Honegger

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