Cross-linked bromelain inhibits lipopolysaccharide-induced cytokine production involving cellular signaling suppression in rats

Journal of Agricultural and Food Chemistry
Rolis Chien-Wei HouKee-Ching G Jeng

Abstract

Bromelain has been reported to have anti-inflammatory and immunomodulatory effects. It has been cross-linked with organic acids and polysaccharides by gamma irradiation. The cross-linked (CL)-bromelain preparation resisted an acidic environment of pH 3 for 2 h and preserved 80% of its enzyme activity. Pretreatment of rats with CL-bromelain intragastrically for 7 days significantly reduced serum cytokine production induced by injected i.p. with 2.5 mg/kg of lipopolysaccharide (LPS). Bromelain significantly reduced serum glutamate-oxalacetate transaminase induced by LPS. The anti-inflammatory effect of CL-bromelain was correlated with reduced LPS-induced NF-kappaB activity and cyclooxygenase 2 (COX-2) mRNA expression in rat livers. In addition, CL-bromelain dose-dependently inhibited LPS-induced COX-2 mRNA and prostaglandin E2 (PGE2) in BV-2 microglial cells. CL-Bromelain also suppressed the LPS-activated extracellular signal-regulated kinase (ERK), c-Jun N-terminal kinase (JNK), and p38 mitogen-activated protein kinase (MAPK). In conclusion, the anti-inflammatory effects of the CL-bromelain preparation in vivo and in vitro suggest its therapeutic potentials.

References

Jan 1, 1996·Annual Review of Immunology·A S Baldwin
Aug 16, 2001·American Journal of Physiology. Lung Cellular and Molecular Physiology·R C ReddyT J Standiford
Aug 18, 2001·The Journal of Biological Chemistry·B Poligone, A S Baldwin
Oct 2, 2001·Cellular and Molecular Life Sciences : CMLS·H R Maurer
Jan 11, 2002·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·J LeipnerR Saller
Feb 18, 2003·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·A F WalkerR W Middleton
Nov 1, 1962·Angiology·B SELIGMAN
Mar 30, 2005·Multiple Sclerosis : Clinical and Laboratory Research·U BaumhacklJ Mertin
Apr 21, 2005·Evidence-based Complementary and Alternative Medicine : ECAM·Sarah BrienDick Middleton
May 12, 2005·International Journal of Pharmaceutics·Mika HoriYoshiharu Machida

❮ Previous
Next ❯

Citations

Sep 24, 2013·BMC Complementary and Alternative Medicine·Thomas Mohr, Lucia Desser
Apr 11, 2013·Cancer Investigation·Krishna PillaiDavid Lawson Morris
Aug 25, 2009·Cancer Letters·Katya ChobotovaFadzilah Adibah Abdul Majid
Mar 31, 2015·Journal of Bioscience and Bioengineering·Chunyeh ChenYoumin Shieh
Feb 11, 2014·Carbohydrate Polymers·Jianguo WangTianli Yue
Jan 11, 2013·Biotechnology Research International·Rajendra PavanAjay Kumar
Sep 8, 2016·Biomedical Reports·Vidhya RathnaveluRajendran Ramesh
Aug 14, 2021·International Journal of Immunopathology and Pharmacology·Roberta BottegaGiovanni Di Lorenzo

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.