PMID: 9182735Jun 1, 1997Paper

Cross-linking analysis of the ryanodine receptor and alpha1-dihydropyridine receptor in rabbit skeletal muscle triads

The Biochemical Journal
B E Murray, K Ohlendieck

Abstract

In mature skeletal muscle, excitation-contraction (EC) coupling is thought to be mediated by direct physical interactions between the transverse tubular, voltage-sensing dihydropyridine receptor (DHPR) and the ryanodine receptor (RyR) Ca2+ release channel of the sarcoplasmic reticulum (SR). Although previous attempts at demonstrating interactions between purified RyR and alpha1-DHPR have failed, the cross-linking analysis shown here indicates low-level complex formation between the SR RyR and the junctional alpha1-DHPR. After cross-linking of membranes highly enriched in triads with dithiobis-succinimidyl propionate, distinct complexes of more than 3000 kDa were detected. This agrees with numerous physiological and electron-microscopic findings, as well as co-immunoprecipitation experiments with triad receptors and receptor domain-binding studies. However, a distinct overlap of immunoreactivity between receptors was not observed in crude microsomal preparations, indicating that the triad complex is probably of low abundance. Disulphide-bonded, high-molecular-mass clusters of triadin, the junctional protein proposed to mediate interactions in triads, were confirmed to be linked to the RyR. Calsequestrin and the SR Ca2+-ATPase we...Continue Reading

Citations

Jan 18, 2002·Journal of Applied Physiology·Kevin CulliganKay Ohlendieck
Jul 28, 2001·Clinical and Experimental Pharmacology & Physiology·G S Posterino
Sep 17, 2002·European Journal of Biochemistry·Louise GloverKay Ohlendieck
May 17, 2007·Orphanet Journal of Rare Diseases·Heinz Jungbluth
Oct 25, 2011·The Journal of Biological Chemistry·Lucia GoliniVincenzo Sorrentino
Aug 13, 2005·Journal of Muscle Research and Cell Motility·A DivetF Zorzato
Sep 6, 2005·Biochimica Et Biophysica Acta·Pamela DonoghueKay Ohlendieck
Nov 30, 1999·Molecular Cell Biology Research Communications : MCBRC·N J LennonK Ohlendieck
May 18, 2000·Biochemical and Biophysical Research Communications·L K BrennanK Ohlendieck
Jul 5, 2001·Biochemical and Biophysical Research Communications·J J MackrillT V McCarthy
Dec 17, 1997·Biochemical and Biophysical Research Communications·P B MaguireK Ohlendieck
Aug 26, 1998·Biochemical and Biophysical Research Communications·D M FinnK Ohlendieck
Aug 12, 1999·Biochemical and Biophysical Research Communications·G R FroemmingK Ohlendieck
Mar 27, 2001·Biochemical and Biophysical Research Communications·K MurataK Nagayama
Apr 21, 1999·Biochimica Et Biophysica Acta·G R FroemmingK Ohlendieck
Feb 13, 1999·European Journal of Pharmacology·G R FroemmingK Ohlendieck
Apr 29, 1998·Trends in Neurosciences·D Walker, M De Waard
Jun 17, 1998·The American Journal of Medicine·J Loke, D H MacLennan
Mar 5, 2003·European Journal of Paediatric Neurology : EJPN : Official Journal of the European Paediatric Neurology Society·Heinz JungbluthFrancesco Muntoni
Sep 25, 2003·Metabolism: Clinical and Experimental·Kay OhlendieckVictor R Preedy

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.