Cross-linking and other structural proteomics techniques: how chemistry is enabling mass spectrometry applications in structural biology

Chemical Science
Alexander Leitner

Abstract

The biological function of proteins is heavily influenced by their structures and their organization into assemblies such as protein complexes and regulatory networks. Mass spectrometry (MS) has been a key enabling technology for high-throughput and comprehensive protein identification and quantification on a proteome-wide scale. Besides these essential contributions, MS can also be used to study higher-order structures of biomacromolecules in a variety of ways. In one approach, intact proteins or protein complexes may be directly probed in the mass spectrometer. Alternatively, various forms of solution-phase chemistry are used to introduce modifications in intact proteins and localizing these modifications by MS analysis at the peptide level is used to derive structural information. Here, I will put a spotlight on the central role of chemistry in such mass spectrometry-based methods that bridge proteomics and structural biology, with a particular emphasis on chemical cross-linking of protein complexes.

References

Mar 1, 1991·Rapid Communications in Mass Spectrometry : RCM·J A LooR D Smith
May 17, 2000·Proceedings of the National Academy of Sciences of the United States of America·M M YoungG Dollinger
Feb 27, 2003·Journal of the American Chemical Society·Michelle Trester-ZedlitzTom W Muir
Feb 24, 2005·Journal of the American Chemical Society·Thomas J D JørgensenPeter Roepstorff
May 20, 2005·Molecular & Cellular Proteomics : MCP·Evgeniy V PetrotchenkoChristoph H Borchers
May 26, 2005·Journal of the American Society for Mass Spectrometry·Joseph A LooIgor V Chernushevich
Nov 3, 2005·Journal of the American Society for Mass Spectrometry·David M Hambly, Michael L Gross
Aug 12, 2006·Expert Review of Proteomics·David RobinetteChristoph H Borchers
Sep 15, 2006·Proteomics·Alexander Leitner, Wolfgang Lindner
Jan 4, 2008·Journal of the American Chemical Society·Kasper D RandThomas J D Jørgensen
Mar 15, 2008·Analytical Chemistry·Vanessa Leah Mendoza, Richard W Vachet
Jul 5, 2008·Nature Protocols·Brandon T RuotoloCarol V Robinson
Aug 5, 2008·Analytical Chemistry·Thomas E WalesJohn R Engen
Nov 19, 2008·Mass Spectrometry Reviews·Vanessa Leah Mendoza, Richard W Vachet
Jul 2, 2009·Molecular & Cellular Proteomics : MCP·Alessandro OriDavid G Fernig
Aug 27, 2009·Proceedings of the National Academy of Sciences of the United States of America·Thomas E AngelMark R Chance
Jul 14, 2010·Molecular & Cellular Proteomics : MCP·Evgeniy V PetrotchenkoChristoph H Borchers
Aug 24, 2010·Mass Spectrometry Reviews·Evgeniy V Petrotchenko, Christoph H Borchers
Dec 22, 2010·Chemical Society Reviews·Lars KonermannYu-Hong Liu
Jun 28, 2011·Current Opinion in Structural Biology·Lars KonermannBradley B Stocks
Sep 8, 2011·Analytical Chemistry·Lisa M JonesMichael L Gross
Nov 1, 2011·Journal of the American Society for Mass Spectrometry·Michael GötzeAndrea Sinz
Nov 10, 2011·Molecular Systems Biology·Nagarjuna NagarajMatthias Mann
Nov 10, 2011·Molecular Systems Biology·Martin BeckRuedi Aebersold
Jan 31, 2012·Molecular & Cellular Proteomics : MCP·Alexander LeitnerRuedi Aebersold
Feb 7, 2012·Proceedings of the National Academy of Sciences of the United States of America·Keren LaskerWolfgang Baumeister
Feb 10, 2012·Journal of the Royal Society, Interface·Gillian R Hilton, Justin L P Benesch
Feb 14, 2012·Rapid Communications in Mass Spectrometry : RCM·Romy FritzscheAndrea Sinz
Mar 13, 2012·Analytica Chimica Acta·Andrew J PercyDavid C Schriemer
Mar 13, 2012·International Journal of Mass Spectrometry·Hao ZhangMichael L Gross

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Citations

Sep 30, 2017·Proteomics. Clinical Applications·Henry A AdeolaNonhlanhla P Khumalo
Jul 9, 2020·Computational and Structural Biotechnology Journal·Seungjin Na, Eunok Paek
Mar 17, 2021·Analytical Methods : Advancing Methods and Applications·Kevin M Downard
Jun 2, 2021·Critical Reviews in Food Science and Nutrition·Sara BenedéElena Molina
Feb 23, 2019·Journal of Proteome Research·Zixiang FangSaiful M Chowdhury
May 7, 2019·Organic Letters·Yuanwei DaiQiang Zhang

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Methods Mentioned

BETA
X-ray
NMR
electron microscopy
protein folding
footprinting
affinity purification
gel filtration

Software Mentioned

HDX
MS
Mass Spec Studio
XL

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