PMID: 9188846Feb 1, 1997Paper

Cross-linking CTX, a novel thymocyte-specific molecule, inhibits the growth of lymphoid tumor cells in Xenopus

Molecular Immunology
Jacques RobertL Du Pasquier

Abstract

CTX, a new Xenopus Ig superfamily molecule present on some cortical thymocytes and lymphoid tumor cells, is expressed at the cell surface under six differently glycosylated isoforms as shown by two-dimensional gel analysis and by endo F glycosidase treatment. Following chemical cross-linking before immunoprecipitation, a large fraction of surface CTX forms non-covalently linked dimers at the cell surface. This finding, which is consistent with the presence of a J segment with diglycine beta bulge in the V region of the molecule, suggests that this dimer has the same conformation as a T-cell receptor (TCR) or an Ig molecule. The V8 digest patterns of the monomers and dimers are identical. While this suggests that the dimer is a homodimer of two CTX chains, it does not distinguish whether each CTX chain is encoded by the same or different gene loci. When tumor cells were added to culture wells that had been coated with the anti-CTX monoclonal antibody X71, 30-50% underwent rapid (within 30 min) morphological changes followed by growth inhibition as determined by a decrease in thymidine incorporation and by direct cell counting. No apoptosis, calcium flux or external calcium requirement was noted after cross-linking of CTX. These ...Continue Reading

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Citations

Dec 23, 1998·European Journal of Immunology·I ChrétienL Du Pasquier
Jun 8, 1999·European Journal of Immunology·L Du PasquierI Chrétien
Jan 7, 1999·Developmental and Comparative Immunology·J Robert, N Cohen
Jan 23, 1999·Immunological Reviews·J Robert, N Cohen
Aug 15, 2014·Cellular and Molecular Life Sciences : CMLS·Eva-Stina EdholmJacques Robert
Aug 28, 2004·Comptes rendus biologies·Louis Du Pasquier
Jun 18, 2004·Immunological Reviews·Louis Du PasquierRosaria De Santis
Jun 21, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·H Travis IchikawaAndrea Bottaro

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