Cross-linking of G-proteins to the prolactin receptor in rat NB2 lymphoma cells

Biochemical and Biophysical Research Communications
C K TooH G Friesen

Abstract

In Nb2 cell membranes, two guanine nucleotide-binding protein (G-protein) species (Mr 43.5 and 46.5 kD) were [32P]-ADP-ribosylated by cholera toxin, while a single protein (Mr 41.5 kD) was [32P]-ADP-ribosylated by pertussis toxin. Immunostaining indicated two immunoreactive prolactin (PRL) receptor moieties of 56 and 64 kD. Cross-linking with ethylene glyco bis[succinimidyl-succinate] (mol. length of 16.1 A) generated a high mol. wt., [32P]-ADP-ribosylated band of 140-160 kD which also showed immunoreactivity with antiserum to the PRL receptor. Other cross-linkers with shorter molecular lengths (8.6 - 11.4 A) were ineffective. These findings indicate that the Nb2 lactogen receptor is complexed with G-proteins and provide evidence for the role of G-proteins in mediating PRL-stimulated mitognesis in Nb2 cells.

References

Apr 30, 1990·Biochemical and Biophysical Research Communications·R ZhangM L Dufau
Aug 1, 1990·Molecular Endocrinology·M ShirotaP A Kelly
Mar 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·M EderyJ Djiane

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Citations

Mar 1, 1995·Endocrine·J L Larsen, T W Burkman
Mar 14, 2000·Archives of Medical Research·F LarreaA Ulloa-Aguirre
May 21, 1998·Antisense & Nucleic Acid Drug Development·C K Too
Sep 6, 2007·Endocrine Journal·Sukanya JaroenpornKazuyoshi Taya
Aug 30, 1991·Biochemical and Biophysical Research Communications·J G Seehafer, A R Shaw
Oct 6, 2011·Nature Reviews. Urology·Vincent GoffinMarja T Nevalainen
Jun 2, 1995·The Journal of Biological Chemistry·C V ClevengerA M Gewirtz

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