PMID: 697574Jan 1, 1978Paper

Cross-linking of Newcastle disease virus (NDV) proteins

Archives of Virology
Y NagaiT Matsumoto

Abstract

The proxomity and spatial relationships of the structural proteins of Newcastle disease virus (NDV) were studied by chemical cross-linking with a series of imidoesters. When the virions were reacted by the cross-linker with a distance 6.1A or longer between the functional groups and analyzed by polyacrylamide gel electrophoresis, remarkable changes were observed in the migration patterns of the viral proteins. The most striking one was the extensive decrease in the intensity of the M protein band, and although not so strikingly, glycoprotein and nucleocapsid protein bands were reduced significantly. Instead, several protein complexes appeared at and near the top of the gels. The protein complexes formed by a reversible cross-linker, dimethyl-3,3'-dithiobispropionimidate (DTBP), were analyzed by two dimensional electrophoresis; the complexes on the first-dimension cylindrical gels were cleaved by reduction with 2-mercaptoethanol and electrophoresed laterally on the second-dimension slab gels. The results indicated that homodimers of glycoprotein, nucleocapsid protein and M protein were generated under the condition of the most gentle cross-linking employed. At the same time, however, trimer and higher homopolymers of M protein w...Continue Reading

References

Dec 1, 1975·Journal of Virology·L E HightowerM A Bratt
Nov 1, 1974·The Journal of General Virology·N F Moore, D C Burke
Oct 1, 1974·Proceedings of the National Academy of Sciences of the United States of America·H Garoff, K Simons
Feb 1, 1973·Journal of Virology·A Scheid, P W Choppin
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey
Jul 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·G E Davies, G R Stark

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Citations

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·A HiranoT C Wong
Jan 19, 2007·Journal of Virology·Takeshi NodaYoshihiro Kawaoka
Jan 1, 1982·Microbiology and Immunology·T Matsumoto

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