PMID: 4074749Dec 20, 1985Paper

Cross-linking studies of steroidogenic electron transfer: covalent complex of adrenodoxin reductase with adrenodoxin

Biochimica Et Biophysica Acta
S A UsanovA A Akhrem

Abstract

Bifunctional reagents 3,3'-dithiobis(succinimidyl propionate), 1-ethyl 3-(3-dimethylaminopropyl)carbodiimide and N-succinimidyl 3-(2-pyridyldithio)propionate have been used in an attempt to study molecular organization and covalent cross-linking of adrenodoxin reductase with adrenodoxin, the components of steroidogenic electron transfer system in bovine adrenocortical mitochondria. There was no cross-linking of individual proteins by the bifunctional reagents used, except for adrenodoxin cross-linking with water-soluble carbodiimide. Substantial cross-linking of adrenodoxin reductase with adrenodoxin was observed when water-soluble carbodiimide was used as cross-linking reagent. However, the cross-linked complex failed to transfer electrons. Significant amounts of the functional cross-linked complex (up to 42%) were observed when the proteins were cross-linked with N-succinimidyl 3-(2-pyridyldithio)propionate. Using gel filtration, ion-exchange chromatography and affinity chromatography on adrenodoxin-Sepharose, the complex was obtained in a highly purified form. In the presence of cytochrome P-450scc or cytochrome c, the cross-linked complex of adrenodoxin reductase with adrenodoxin was active in electron transfer from NADPH t...Continue Reading

References

Jul 25, 1977·Biochemical and Biophysical Research Communications·M KatagiriK Suhara
Jul 29, 1983·Biochimica Et Biophysica Acta·L M GerenF Millett
Nov 9, 1984·Biochimica Et Biophysica Acta·S A UsanovA A Akhrem
Nov 15, 1983·Biochemical and Biophysical Research Communications·D J Davis, K Hough
Apr 10, 1980·The Journal of Biological Chemistry·I Hanukoglu, C R Jefcoate
May 28, 1982·Molecular and Cellular Biochemistry·J D LambethH Kamin

Citations

Sep 1, 1992·Archives of Biochemistry and Biophysics·D A BironaitéS A Usanov
Aug 30, 1991·Biochemical and Biophysical Research Communications·M Takeshima, T Hara
Jan 6, 2000·Archives of Biochemistry and Biophysics·I A PikulevaY Kim

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