Cross-neutralization of SARS-CoV-2 by a human monoclonal SARS-CoV antibody.

Nature
Dora PintoDavide Corti

Abstract

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is a newly emerged coronavirus that is responsible for the current pandemic of coronavirus disease 2019 (COVID-19), which has resulted in more than 3.7 million infections and 260,000 deaths as of 6 May 20201,2. Vaccine and therapeutic discovery efforts are paramount to curb the pandemic spread of this zoonotic virus. The SARS-CoV-2 spike (S) glycoprotein promotes entry into host cells and is the main target of neutralizing antibodies. Here we describe several monoclonal antibodies that target the S glycoprotein of SARS-CoV-2, which we identified from memory B cells of an individual who was infected with severe acute respiratory syndrome coronavirus (SARS-CoV) in 2003. One antibody (named S309) potently neutralizes SARS-CoV-2 and SARS-CoV pseudoviruses as well as authentic SARS-CoV-2, by engaging the receptor-binding domain of the S glycoprotein. Using cryo-electron microscopy and binding assays, we show that S309 recognizes an epitope containing a glycan that is conserved within the Sarbecovirus subgenus, without competing with receptor attachment. Antibody cocktails that include S309 in combination with other antibodies that we identified further enhanced SARS-CoV-2 ...Continue Reading

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Datasets Mentioned

BETA
MN908947

Methods Mentioned

BETA
enzyme-linked immunosorbent assay
biolayer interferometry
flow cytometry
biosensors
Assay
transfection
X-ray

Software Mentioned

Biostrings
EMringer
Phenix
TreeStar
MAFFT
Relion
Warp
Bioconductor
REFMAC5
PRISM

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