Jan 20, 1976

Cross partition and determination of net charge of the isoenzymes of enolase

Biochimica Et Biophysica Acta
G Blomquist


Enolase from bakers' yeast was separated into three isoenzymes by countercurrent distribution. The isoenzymes were partitioned in aqueous polymer two-phase systems containing positively charged trimethylamino poly(ethylene glycol) or negatively charged poly(ethylene glycol) sulphonate. The plots of the partition coefficient of each isoenzyme versus pH in the two biphasic systems intersect at pH equal to the isoelectric point. From slopes of the plots, the net charge of the isoenzymes at pH 6.57 was determined to be +2, -3, and -8 respectively.

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Mentioned in this Paper

Ethylene Glycol Measurement
Countercurrent Chromatography
Ethylene glycol
Bos indicus
Saccharomyces cerevisiae
Serum Albumin, Bovine
Hydrogen-Ion Concentration

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