Cross-priming isothermal amplification combined with nucleic acid test strips for detection of meat species

Analytical Biochemistry
Fangyuan ZhengJiarong Pan

Abstract

Adulteration of high-quality meat with their cheaper counterparts can be minimized by rapid and reliable methods for detecting meat species. Here an isothermal cross-primer amplification (CPA) technique combined with colloidal gold nucleic acid test strips (CPA strips) was developed to differentiate cow, sheep, arctic fox, and pig meat. A simple primer design for multiplex differentiation using a universal single-labeled CPA primer system and four detection-level species-specific labeling primers were analyzed by colloidal gold-based test strip assay. Moreover, simultaneous detection of fox and pig meat on a double-test line strip was feasible. The CPA strip assay indicated a lower amounts sensitivity of 0.3 ng DNA when one targeted species was tested and a detection limit of 1% when arctic fox meat was detected in the meat mixtures. Using a minimal set of primers, this study provides a promising tool for detecting the species of different types of meat using a constant temperature amplification technology.

References

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Oct 31, 2018·Food Chemistry·Maria MagiatiDespina P Kalogianni

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Citations

Jan 15, 2021·Comprehensive Reviews in Food Science and Food Safety·Yogesh Kumar, Kairam Narsaiah
May 20, 2021·Journal of Agricultural and Food Chemistry·Panting SangHe Qian
May 24, 2021·Annals of Clinical Microbiology and Antimicrobials·Shuang WuQinghua Hu

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