Cross-Reactive and Potent Neutralizing Antibody Responses in Human Survivors of Natural Ebolavirus Infection

Cell
Andrew I FlyakJames E Crowe

Abstract

Recent studies have suggested that antibody-mediated protection against the Ebolaviruses may be achievable, but little is known about whether or not antibodies can confer cross-reactive protection against viruses belonging to diverse Ebolavirus species, such as Ebola virus (EBOV), Sudan virus (SUDV), and Bundibugyo virus (BDBV). We isolated a large panel of human monoclonal antibodies (mAbs) against BDBV glycoprotein (GP) using peripheral blood B cells from survivors of the 2007 BDBV outbreak in Uganda. We determined that a large proportion of mAbs with potent neutralizing activity against BDBV bind to the glycan cap and recognize diverse epitopes within this major antigenic site. We identified several glycan cap-specific mAbs that neutralized multiple ebolaviruses, including SUDV, and a cross-reactive mAb that completely protected guinea pigs from the lethal challenge with heterologous EBOV. Our results provide a roadmap to develop a single antibody-based treatment effective against multiple Ebolavirus infections.

References

Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·A SanchezS T Nichol
Jan 1, 1996·Journal of Structural Biology·M van HeelM Schatz
Sep 5, 1998·The Journal of Infectious Diseases·M BrayJ Huggins
Jul 21, 2004·Journal of Computational Chemistry·Eric F PettersenThomas E Ferrin
Apr 16, 2005·Science·Kartik ChandranJames M Cunningham
Jul 25, 2006·Journal of Structural Biology·Guang TangSteven J Ludtke
Jan 24, 2007·PLoS Pathogens·Heinz FeldmannThomas W Geisbert
Jun 3, 2008·Journal of Immunological Methods·Xiaocong YuJames E Crowe
Nov 22, 2008·PLoS Pathogens·Jonathan S TownerStuart T Nichol
Mar 6, 2009·Journal of Structural Biology·Gabriel C LanderBridget Carragher
Jun 23, 2009·Genome Research·Martin KrzywinskiMarco A Marra
Jul 27, 2010·Reviews in Medical Virology·Thomas W GeisbertHeinz Feldmann
Jun 3, 2011·Cold Spring Harbor Protocols·Véronique GiudicelliMarie-Paule Lefranc
Jun 15, 2011·PLoS Neglected Tropical Diseases·Adam MacneilPierre E Rollin
Sep 20, 2011·Clinical Immunology : the Official Journal of the Clinical Immunology Society·Xiangguo QiuSteven M Jones
Jun 16, 2012·Science Translational Medicine·Xiangguo QiuGary P Kobinger
Sep 25, 2012·Journal of Structural Biology·Sjors H W Scheres
Oct 17, 2012·Proceedings of the National Academy of Sciences of the United States of America·Gene Garrard OlingerLarry Zeitlin
Dec 29, 2012·PLoS Pathogens·Gopi S MohanChinglai Yang
Nov 19, 2014·Proceedings of the National Academy of Sciences of the United States of America·Charles D MurinErica Ollmann Saphire
Feb 28, 2015·Cell·Andrew I FlyakJames E Crowe
Feb 28, 2015·Cell·Takao HashiguchiErica Ollmann Saphire
May 23, 2015·The Journal of Infectious Diseases·Humberto HernandezBarry Rockx
Jul 15, 2015·The Journal of Clinical Investigation·Michelle MeyerAlexander Bukreyev
Aug 28, 2015·Journal of Virology·Edgar DavidsonBenjamin J Doranz

❮ Previous
Next ❯

Citations

Apr 23, 2016·Database : the Journal of Biological Databases and Curation·Karina YusimBette Korber
May 18, 2016·Scientific Reports·Qi ZhangLinqi Zhang
Jun 25, 2016·Trends in Microbiology·Erica Ollmann Saphire, M Javad Aman
Aug 27, 2016·Nature Microbiology·Jesper PallesenAndrew B Ward
Sep 28, 2016·Expert Opinion on Investigational Drugs·A L MoekotteM P Grobusch
Dec 3, 2016·The Journal of General Virology·Magdalena Formella, Derek Gatherer
Apr 12, 2017·Scientific Reports·Daniel R Beniac, Timothy F Booth
Jan 1, 2016·Uirusu·Wakako Furuyama, Ayato Takada
Jan 31, 2018·Nature Reviews. Immunology·Laura M Walker, Dennis R Burton
Mar 4, 2018·The Journal of Biological Chemistry·Elisabeth K NyakaturaJonathan R Lai
Oct 8, 2017·Protein Science : a Publication of the Protein Society·Melanie H DietrichThilo Stehle
Aug 18, 2016·Emerging Microbes & Infections·Jennifer LambourMireia Pelegrin
Jun 26, 2018·The Journal of Infectious Diseases·Kelly L WarfieldM Javad Aman
Aug 24, 2018·PLoS Pathogens·Philipp A IlinykhAlexander Bukreyev
May 27, 2016·Clinical and Vaccine Immunology : CVI·Jing Jin, Graham Simmons
Jan 29, 2016·Journal of Virology·Philipp A IlinykhAlexander Bukreyev
Feb 8, 2019·Journal of Virology·Lauren E WilliamsonJames E Crowe
Jul 10, 2018·The Journal of Infectious Diseases·Kerstin FischerSandra Diederich
Jul 10, 2018·The Journal of Infectious Diseases·Pavlo GilchukJames E Crowe
Jan 10, 2020·Virologica Sinica·Na LiSheng Cao
Apr 12, 2017·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Teresa LambeKatie J Ewer
Oct 9, 2019·Nature Medicine·Stefanie A EhrhardtFlorian Klein
Apr 19, 2019·Nature Communications·Liam B KingErica Ollmann Saphire
Jan 5, 2017·Bulletin de la Société de pathologie exotique·F GallaisL Bellanger
Jul 21, 2017·The Journal of Experimental Medicine·Spencer W StonierJohn M Dye
Aug 15, 2018·The Journal of Infectious Diseases·Matthew S BrambleAnne W Rimoin
Aug 28, 2018·Frontiers in Immunology·Kuldeep DhamaWanpen Chaicumpa
Oct 8, 2019·Human Vaccines & Immunotherapeutics·John J Suschak, Connie S Schmaljohn

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell eTOC

Cell is a scientific journal publishing research across a broad range of disciplines within the life sciences field. Discover the latest research from Cell here.