Cross-reactivity and expansion of dengue-specific T cells during acute primary and secondary infections in humans.

Scientific Reports
Heather FribergAnuja Mathew

Abstract

Serotype-cross-reactive memory T cells responding to secondary dengue virus (DENV) infection are thought to contribute to disease. However, epitope-specific T cell responses have not been thoroughly compared between subjects with primary versus secondary DENV infection. We studied CD8(+) T cells specific for the HLA-A*1101-restricted NS3(133) epitope in a cohort of A11(+) DENV-infected patients throughout acute illness and convalescence. We compared the expansion, serotype-cross-reactivity, and activation of these cells in PBMC from patients experiencing primary or secondary infection and mild or severe disease by flow cytometry. Our results show expansion and activation of DENV-specific CD8(+) T cells during acute infection, which are predominantly serotype-cross-reactive regardless of DENV infection history. These data confirm marked T cell activation and serotype-cross-reactivity during the febrile phase of dengue; however, A11-NS3(133)-specific responses did not correlate with prior antigenic exposure or current disease severity.

References

Feb 1, 1990·The American Journal of Tropical Medicine and Hygiene·M G GuzmánL Morier
Jan 1, 1988·The American Journal of Tropical Medicine and Hygiene·D S BurkeR M Scott
May 1, 1997·The American Journal of Tropical Medicine and Hygiene·S TheinJ Aaskov
Aug 1, 1997·The Journal of Infectious Diseases·S KalayanaroojF A Ennis
Aug 1, 1997·The Journal of Infectious Diseases·D W VaughnA Nisalak
Sep 29, 1999·Journal of Medical Virology·S GreenF A Ennis
Oct 9, 1999·The Journal of Infectious Diseases·S GreenF A Ennis
Dec 23, 1999·The Journal of Infectious Diseases·D W VaughnA Nisalak
May 10, 2002·The Journal of Infectious Diseases·Daniel H LibratyAlan L Rothman
May 23, 2002·The Journal of Immunology : Official Journal of the American Association of Immunologists·Iva ZivnaAlan L Rothman
Jul 18, 2002·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Beatríz SierraMaría G Guzmán
Jun 17, 2003·Nature Medicine·Juthathip MongkolsapayaGavin Screaton
May 4, 2004·Journal of Medical Virology·Ana B PérezMaría G Guzmán
Aug 6, 2005·The Journal of Immunology : Official Journal of the American Association of Immunologists·Maloy M Mangada, Alan L Rothman
Feb 24, 2006·The Journal of Immunology : Official Journal of the American Association of Immunologists·Hema S BashyamAlan L Rothman
Aug 10, 2006·The Journal of Infectious Diseases·Khin S MyintDaniel H Libraty
Apr 25, 2007·The Journal of Experimental Medicine·Kerstin LühnSarah Rowland-Jones
Apr 30, 2009·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·Melanie WieselAnnette Oxenius
Oct 6, 2009·Current Topics in Microbiology and Immunology·Alan L Rothman
Nov 26, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Rama S AkondyRafi Ahmed
Apr 28, 2010·Immunology and Cell Biology·Heather FribergAnuja Mathew
May 21, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Nguyen Thi Phuong DungCameron P Simmons
Jun 12, 2010·Immunological Reviews·Raymond M WelshLiisa K Selin
Sep 15, 2010·Proceedings of the National Academy of Sciences of the United States of America·Thaneeya DuangchindaGavin Screaton

❮ Previous
Next ❯

Citations

Dec 15, 2012·Critical Reviews in Immunology·Galina PetrovaJack Gorski
Jun 3, 2014·Current Tropical Medicine Reports·Alan L RothmanJeffrey R Currier
Nov 3, 2014·Annual Review of Virology·Theodore C Pierson, Michael S Diamond
Aug 26, 2015·Neuropathology : Official Journal of the Japanese Society of Neuropathology·Barbara Cristina Baldez VasconcelosDaniel Guerreiro Diniz
Oct 16, 2015·Vaccine·Cameron P SimmonsBridget Wills
Nov 13, 2015·Expert Review of Vaccines·Laura Rivino
Jul 28, 2012·Reviews in Medical Virology·Pd Griffiths
Nov 26, 2015·Nature Reviews. Immunology·Gavin ScreatonCatherine Roberts
Nov 13, 2015·Expert Review of Vaccines·Anon Srikiatkhachorn, In-Kyu Yoon
Mar 18, 2015·Journal of Medical Virology·Zhiliang DuanJinsheng Wen
Mar 13, 2015·Science Translational Medicine·Laura RivinoPaul A MacAry
Dec 19, 2014·Journal of the Royal Society, Interface·Rotem Ben-Shachar, Katia Koelle
Jun 1, 2016·The Journal of Experimental Medicine·Lance TurtleTom Solomon
Nov 12, 2014·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Max S ToppRalf C Bargou
Nov 18, 2016·PLoS Computational Biology·Rotem Ben-ShacharKatia Koelle
Jun 20, 2018·The Journal of Infectious Diseases·Kirk HaltaufderhydeAnuja Mathew
Aug 5, 2017·PLoS Pathogens·Michael K McCrackenStephen J Thomas
Jan 25, 2019·Expert Review of Vaccines·Laura LazoLázaro Gil
Sep 22, 2019·Bulletin of Mathematical Biology·Milen BorisovPeter Rashkov
Aug 22, 2015·PLoS Neglected Tropical Diseases·Sangay ZangmoStefan Fernandez
Mar 1, 2017·Future Virology·Joris SprokholtTeunis Bh Geijtenbeek
Sep 26, 2019·Frontiers in Immunology·Yuan TianDaniela Weiskopf
Apr 22, 2020·Virologica Sinica·Edith Marcial-JuárezLeopoldo Flores-Romo
Jan 27, 2019·Nature Reviews. Immunology·Ashley L St John, Abhay P S Rathore
Dec 18, 2018·PLoS Neglected Tropical Diseases·Heather FribergAlan L Rothman
Nov 17, 2020·Frontiers in Cellular and Infection Microbiology·Rahul ShuklaNavin Khanna
Nov 24, 2020·Frontiers in Cellular and Infection Microbiology·Christine A KingTimothy P Endy
Nov 19, 2020·Current Treatment Options in Infectious Diseases·Vijaya Satchidanandam

❮ Previous
Next ❯

Methods Mentioned

BETA
flow cytometry

Software Mentioned

FlowJo
GraphPad Prism
Stata Intercooled

Related Concepts

Related Feeds