PMID: 6166719Jun 1, 1981Paper

Cross-reactivity between C-reactive protein and idiotypic determinants on A phosphocholine-binding murine myeloma protein

The Journal of Experimental Medicine
J E Volanakis, J F Kearney

Abstract

Binding of human 125I-C-reactive protein (CRP) to sheep erythrocytes sensitized with pneumococcal C polysaccharide (E-PnC) was found to be Ca++ dependent and inhibitable by phosphocholine, CRP, and HOPC 8. Binding of 125I-HOPC 8 to EPnC was Ca++ -independent but could also be inhibited by phosphocholine, CRP, and HOPC 8. Thus, CRP and HOPC 8, despite a differential Ca++ requirement, share a common binding specificity for phosphocholine. A monoclonal anti-idiotypic antibody (MAB), GB4-10, prepared in A/J mice immunized with BALB/c HOPC 8 inhibited the binding of both 125I-CRP and 125I-HOPC 8 to E-PnC. In addition, both proteins bound to GB4-10 immobilized on polysterene tubes. Interestingly, binding of 125I-CRP to GB4-10 required Ca++. Similar results were also obtained with another MAB (AB1-2) prepared similarly to GB4-10, whereas neither protein bound to a control MAB (EB3-7) against an alpha1 leads to 3 dextran-binding myeloma protein, J558. Binding of 125I-HOPC 8 to GB4-10 could be inhibited by HOPC 8, keyhole limpet hemocyanin-phosphocholine but not phosphocholine but not phosphocholine, and in the presence of Ca++ by CRP. These data indicate that CRP bears antigenic determinants cross-reacting with certain idiotypic determ...Continue Reading

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Citations

Apr 13, 2001·Journal of Molecular Recognition : JMR·J J MarchalonisA B Edmundson
Jan 1, 1982·Immunogenetics·M SuzanB Rubin
Oct 20, 1998·Biulleten' eksperimental'noĭ biologii i meditsiny·P G NazarovA D Denisenko
Mar 16, 1984·Journal of Immunological Methods·S R BarnumJ E Volanakis
May 25, 1988·Journal of Immunological Methods·T J OglesbyJ E Volanakis
Jan 1, 1989·Developmental and Comparative Immunology·J J Marchalonis, S F Schluter
Jul 1, 1992·Molecular Immunology·L Saunero-NavaT W Du Clos
Jul 1, 1993·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·J J MarchalonisS F Schluter
Oct 7, 2011·Chemical Communications : Chem Comm·Eunjoo KimSang Won Jeong
Feb 1, 1982·The Journal of Experimental Medicine·A F Clark, J D Capra
Apr 1, 1984·The Journal of Experimental Medicine·G R VastaH Kohler
Jun 1, 1990·The Journal of Experimental Medicine·A PuccettiR S Schwartz
Jan 1, 1982·Immunological Communications·J F KearneyJ E Volanakis
Jan 1, 1990·Medical Microbiology and Immunology·S NishinaritaT Horie
May 1, 1989·Journal of Cellular Biochemistry·S J SwansonR F Mortensen

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