Cross-reactivity between cockroach and ladybug using the radioallergosorbent test

Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology
Matthew T ClarkWilliam Dolen

Abstract

Home infestations from Harmonia axyridis (ladybug) occur throughout the United States. IgE-mediated sensitization with allergic disease has been reported. The prevalence of ladybug sensitization has been studied by questionnaire and allergy testing in patients diagnosed as having allergic disease. Cross-reactivity with cockroach exists. To determine the prevalence of ladybug specific IgE in the general population by specific IgE immunoassay and to examine cross-reactivity to cockroach. An experimental solid phase for use in immunoassay was prepared using a ladybug extract, and performance characteristics were determined. Serum samples from 100 adult blood bank donors were tested using the ladybug specific IgE immunoassay. Known ladybug IgE-positive serum samples obtained from symptomatic patients were used to study crossreactivity with German cockroach by specific IgE immunoassay inhibition. The mean background response of the assay solid phase was 51 fluorescent units with an analytical cutoff of 59 fluorescent units. It was estimated that a response of 88 fluorescent units corresponds to a specific IgE concentration of 0.1 kUa/L. The extinction dilution curve was linear to 0.1 kUa/L. The assay cutoff was set at 0.1 kUa/L. Of ...Continue Reading

References

Sep 14, 1999·The Journal of Allergy and Clinical Immunology·J A YarbroughW K Dolen
Oct 31, 2006·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Deborah D AlbrightLarry L Hagan
Oct 31, 2006·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·Kusum SharmaHobert L Pence
Feb 13, 2007·The Journal of Allergy and Clinical Immunology·Takuya NakazawaThomas A E Platts-Mills
Mar 30, 2007·Allergy and Asthma Proceedings·David W Goetz
Apr 24, 2008·Allergy and Asthma Proceedings·David W Goetz

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Citations

Apr 19, 2005·Clinical and Molecular Allergy : CMA·Birjis ChinoySami L Bahna
Jul 9, 2011·Immunology and Allergy Clinics of North America·Robert K Bush

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