Abstract
Anti-La (SS-B) antibodies cross-reacting with mouse B1 laminin were reported in sera of patients with systemic lupus erythematosus. However, the common epitope had not been characterized. Immunoblotting conditions were established, allowing detection and elution of anti-La (SS-B)/laminin cross-reacting antibodies. Antibodies adsorbed to mouse B1 laminin represented a subclass of anti-La antibodies. They strongly reacted with human full length recombinant La protein. However, they failed to react with either an N-terminal La peptide consisting of amino acids 1-192 or a C-terminal La peptide starting at methionine 223, while they still reacted with recombinant La peptides consisting of the amino acids 1-341 or starting at 192. These data indicate that the La (SS-B)/laminin epitope is located between amino acids 192-223 of human La protein, which includes the amino acids EAKLRA, common to the nuclear autoantigen La (SS-B) and the human or mouse B1 laminin.
References
Aug 1, 1992·Journal of Autoimmunity·A C Horsfall, L M Rose
Jan 12, 1990·Cell·M BachmannW E Müller
Jan 1, 1990·Nephron·Y ShinkaiS Koshikawa
Feb 1, 1990·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·K BeckJ Engel
Jul 1, 1989·Genes & Development·K MeerovitchN Sonenberg
Apr 11, 1985·Nucleic Acids Research·J Rinke, J A Steitz
Jan 1, 1989·Advances in Immunology·E M Tan
Aug 1, 1989·The Journal of Clinical Investigation·J P BuyonR Winchester
Feb 28, 1985·Journal of Immunological Methods·P R SmithR N Maini
Aug 15, 1970·Nature·U K Laemmli
Dec 1, 1981·Molecular and Cellular Biology·J P HendrickJ A Steitz
Oct 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·J WiluszJ D Keene
Jun 1, 1984·Journal of Virology·M G KurillaJ D Keene
Jan 1, 1984·Cell·J E Stefano
Sep 1, 1995·European Journal of Biochemistry·G J PruijnW J Van Venrooij
Jul 1, 1995·Clinical Immunology and Immunopathology·J García LermaJ Sequi Navarro
Mar 1, 1995·Clinical and Experimental Immunology·J M LiR N Maini
Feb 1, 1994·Journal of Autoimmunity·T DörnerF Hiepe
Jul 1, 1993·Journal of Virology·K MeerovitchN Sonenberg
Dec 1, 1995·Journal of Autoimmunity·H TrösterM Bachmann
Dec 1, 1996·Journal of Autoimmunity·M BachmannD Grölz
May 20, 1997·Gene·D GrölzM Bachmann
Citations
Jul 9, 2002·Immunology·Michael A FourauxGer J M Pruijn
Dec 17, 2005·Arthritis Research & Therapy·Christine FritschSylviane Muller
Oct 27, 2004·Seminars in Arthritis and Rheumatism·Yaniv ShererYehuda Shoenfeld
Jun 28, 2016·Experimental and Therapeutic Medicine·Min Ding, Jianzhong Zhang
Sep 2, 2010·Nano Letters·Richard S GasterShan X Wang