Cross-reactivity of antipneumococcal surface protein C (PspC) antibodies with different strains and evaluation of inhibition of human complement factor H and secretory IgA binding via PspC.

Clinical and Vaccine Immunology : CVI
Adriana T MorenoEliane N Miyaji

Abstract

Pneumococcal surface protein C (PspC) is an important candidate for a cost-effective vaccine with broad coverage against pneumococcal diseases. Previous studies have shown that Streptococcus pneumoniae is able to bind to both human factor H (FH), an inhibitor of complement alternative pathway, and human secretory IgA (sIgA) via PspC. PspC was classified into 11 groups based on variations of the gene. In this work, we used three PspC fragments from different groups (PspC3, PspC5, and PspC8) to immunize mice for the production of antibodies. Immunization with PspC3 induced antibodies that recognized the majority of the clinical isolates as analyzed by Western blotting of whole-cell extracts and flow cytometry of intact bacteria, while anti-PspC5 antibodies showed cross-reactivity with the paralogue pneumococcal surface protein A (PspA), and anti-PspC8 antibodies reacted only with the PspC8-expressing strain. Most of the isolates tested showed strong binding to FH and weaker interaction with sIgA. Preincubation with anti-PspC3 and anti-PspC5 IgG led to some inhibition of binding of FH, and preincubation with anti-PspC3 partially inhibited sIgA binding in Western blotting. The analysis of intact bacteria through flow cytometry show...Continue Reading

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Citations

Dec 28, 2012·Cellular and Molecular Life Sciences : CMLS·Eliane Namie MiyajiPaulo Lee Ho
May 9, 2014·Clinical and Vaccine Immunology : CVI·Cintia F M VadesilhoEliane N Miyaji
Nov 28, 2018·Nature Reviews. Immunology·Maria GeorgievaMarc Lipsitch
Mar 8, 2017·Frontiers in Microbiology·Greiciely O AndreMichelle Darrieux
Mar 3, 2021·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Mehrnaz NouriLars Råberg
Aug 12, 2021·Trends in Molecular Medicine·Bharathi SundareshTim van Opijnen

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