PMID: 9171383Jun 1, 1997Paper

Cross regulation of four GATA factors that control nitrogen catabolic gene expression in Saccharomyces cerevisiae

Journal of Bacteriology
J A CoffmanT G Cooper

Abstract

Nitrogen catabolic gene expression in Saccharomyces cerevisiae has been reported to be regulated by three GATA family proteins, the positive regulators Gln3p and Gat1p/Nil1p and the negative regulator Dal80p/Uga43p. We show here that a fourth member of the yeast GATA family, the Dal80p homolog Deh1p, also negatively regulates expression of some, but not all, nitrogen catabolic genes, i.e., GAP1, DAL80, and UGA4 expression increases in a deh1 delta mutant. Consistent with Deh1p regulation of these genes is the observation that Deh1p forms specific DNA-protein complexes with GATAA-containing UGA4 and GAP1 promoter fragments in electrophoretic mobility shift assays. Deh1p function is demonstrable, however, only when a repressive nitrogen source such as glutamine is present; deh1 delta mutants exhibit no detectable phenotype with a poor nitrogen source such as proline. Our experiments also demonstrate that GATA factor gene expression is highly regulated by the GATA factors themselves in an interdependent manner. DAL80 expression is Gln3p and Gat1p dependent and Dal80p regulated. Moreover, Gln3p and Dal80p bind to DAL80 promoter fragments. In turn, GAT1 expression is Gln3p dependent and Dal80p regulated but is not autogenously regul...Continue Reading

References

Aug 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·J Marine, A Winoto
Apr 23, 1991·European Journal of Biochemistry·D O SpormannD H Wolf
Jul 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·Y H Fu, G A Marzluf
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·L I ZonS H Orkin
Jan 1, 1985·Advances in Microbial Physiology·J M WiameH N Arst
Feb 1, 1988·Journal of Bacteriology·W E Courchesne, B Magasanik
Aug 1, 1987·Journal of Bacteriology·R RaiT G Cooper
Jul 17, 1973·Biochemical and Biophysical Research Communications·R DrillienF Lacroute
Jan 1, 1972·Journal of Bacteriology·R Drillien, F Lacroute
Jan 1, 1983·Methods in Enzymology·R J Rothstein
Jan 1, 1983·Journal of Bacteriology·H ItoA Kimura
Nov 7, 1995·Proceedings of the National Academy of Sciences of the United States of America·H W DetrichL I Zon
Apr 1, 1995·Molecular and Cellular Biology·S XuM C Brandriss

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Citations

Feb 27, 1999·Molecular Microbiology·S Soussi-Boudekou, B André
Aug 7, 1999·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·M WonH S Yoo
Dec 28, 1999·Proceedings of the National Academy of Sciences of the United States of America·J S HardwickS L Schreiber
Mar 4, 2000·The Journal of Biological Chemistry·S ScottT G Cooper
Nov 6, 2009·Molecular Biology of the Cell·Viktor M BoerJoshua D Rabinowitz
Apr 18, 2014·FEMS Yeast Research·Steven V Rødkaer, Nils J Faergeman
May 23, 2015·FEMS Yeast Research·Sviatlana ShashkovaStefan Hohmann
Dec 3, 2017·Molecular Microbiology·Laure Nicolas Annick RiesGustavo H Goldman
Aug 15, 2000·The Journal of Biological Chemistry·P G BertramX F Zheng
Dec 11, 2013·The Journal of Biological Chemistry·Rajendra RaiTerrance G Cooper
Mar 12, 2016·PLoS Computational Biology·Andreas Milias-ArgeitisJohn Lygeros
Mar 13, 2014·Database : the Journal of Biological Databases and Curation·Tzu-Hsien YangWei-Sheng Wu
Jun 6, 2017·FEMS Yeast Research·Terrance G Cooper
Mar 16, 2012·Genetics·Per O Ljungdahl, Bertrand Daignan-Fornier

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