Cross-relaxation bottleneck in water-lysozyme proton magnetization exchange

Biopolymers
J F KakuleH Peemoeller

Abstract

The proton spin-lattice relaxation parameters in natural and deuterated lysozyme solutions have been measured as a function of temperature (0-50 degrees C). The variation of the apparent magnitudes of the water proton magnetizations in the solutions with temperature indicates that magnetic coupling mixes protein and water proton magnetizations. The results are consistent with an exchange cross-relaxation model (Hills, B. P., Mol Phys 1992, 76, 489-508) in which the cross-relaxation acts between the labile and nonlabile protons, rather than between water and protein protons. Although this cross-relaxation pathway clearly affects the observed magnetization fractions in this protein solution, its influence on the relaxation rates is less apparent.

References

Nov 26, 1976·Biochimica Et Biophysica Acta·L Grösch, F Noack
Apr 25, 1986·Nucleic Acids Research·P Marsh
Jun 23, 1970·Biochimica Et Biophysica Acta·B BlicharskaF Noack
Jan 1, 1966·Advances in Protein Chemistry·A Hvidt, S O Nielsen
Mar 21, 1969·Science·I D KuntzG V Purcell
Feb 20, 1993·Journal of Molecular Biology·L J SmithC M Dobson
Apr 17, 1996·Biochimica Et Biophysica Acta·H M Baranowska, K J Olszewski
Jan 1, 1996·Annual Review of Biophysics and Biomolecular Structure·R G Bryant
Aug 29, 2002·Journal of the American Chemical Society·Vladimir P Denisov, Bertil Halle
Jan 14, 2003·Biophysical Journal·Alexandra Van-QuynhRobert G Bryant
Jan 8, 2004·Journal of the American Chemical Society·Kristofer ModigBertil Halle

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Related Papers

Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire
S Prosser, H Peemoeller
Journal of the American Chemical Society
Fabian Vaca ChavezBertil Halle
© 2022 Meta ULC. All rights reserved