Cross-species analysis of the replication complex of Old World arenaviruses reveals two nucleoprotein sites involved in L protein function.

Journal of Virology
Romy KerberStephan Günther

Abstract

Lassa virus (LASV) causing hemorrhagic Lassa fever in West Africa, Mopeia virus (MOPV) from East Africa, and lymphocytic choriomeningitis virus (LCMV) are the main representatives of the Old World arenaviruses. Little is known about how the components of the arenavirus replication machinery, i.e., the genome, nucleoprotein (NP), and L protein, interact. In addition, it is unknown whether these components can function across species boundaries. We established minireplicon systems for MOPV and LCMV in analogy to the existing LASV system and exchanged the components among the three systems. The functional and physical integrity of the resulting complexes was tested by reporter gene assay, Northern blotting, and coimmunoprecipitation studies. The minigenomes, NPs, and L proteins of LASV and MOPV could be exchanged without loss of function. LASV and MOPV L protein was also active in conjunction with LCMV NP, while the LCMV L protein required homologous NP for activity. Analysis of LASV/LCMV NP chimeras identified a single LCMV-specific NP residue (Ile-53) and the C terminus of NP (residues 340 to 558) as being essential for LCMV L protein function. The defect of LASV and MOPV NP in supporting transcriptional activity of LCMV L prote...Continue Reading

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Citations

Apr 5, 2013·Archives of Virology·Frank LennartzWolfgang Garten
Nov 23, 2011·Proceedings of the National Academy of Sciences of the United States of America·Philip J Kranzusch, Sean P J Whelan
Dec 4, 2012·Viruses·Igor S Lukashevich
Jan 25, 2013·Viruses·Juan C Zapata, Maria S Salvato
Dec 22, 2012·Expert Review of Vaccines·Igor S Lukashevich
May 16, 2017·PLoS Pathogens·Maria RosenthalSophia Reindl
Jul 28, 2018·Frontiers in Cellular and Infection Microbiology·Stephen R WelchChristina F Spiropoulou
Aug 26, 2021·Journal of Virology·Jesse D Pyle, Sean P J Whelan

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