Cross-species comparison of gene expression between human and porcine tissue, using single microarray platform--preliminary results

Clinical Transplantation
Gaurang ShahDilip Kittur

Abstract

Xenotransplantation is a potential solution for inadequate supply of donor organs. Pigs are considered the ideal donor for kidney transplantation to human recipients, therefore it is important to understand the gene regulation in the porcine organs. Oligonucleotide array technology has been utilized largely for human, mouse and rat gene expression studies only. Its use with porcine genes has not been reported. We investigated the possibility of studying gene regulation in porcine kidney with a human GeneChip microarray platform. To assess the feasibility of using a single microarrray platform for comparison of expressing data across different species (human and pig), we compared the gene expression profiles of human brain, human kidney and pig kidney using the Affymetrix U-133 A human GeneChip, which contains probes for 22,283 genes. Kidney biopsies from pigs and humans, with normal histology, were used to obtain RNA for porcine and human samples, while a commercially available adult whole cortex total RNA sample (Clontech) was used for the human sample. We assessed the intensity ratio for housekeeping and tissue specific genes. To examine the potential for non-specific binding to create false positive errors in our data, we co...Continue Reading

References

Oct 2, 2002·BMC Genomics·D E MoodyL McIntyre

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Citations

Jul 20, 2007·Reproduction : the Official Journal of the Society for the Study of Fertility·Isabelle GilbertClaude Robert
Oct 1, 2008·Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology·Sophie St-Cyr, Nadia Aubin-Horth
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Sep 8, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Geeta RaiRose G Mage

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