Cross-species protein identification using amino acid composition, peptide mass fingerprinting, isoelectric point and molecular mass: a theoretical evaluation

Journal of Theoretical Biology
M R Wilkins, K L Williams

Abstract

Proteins can be identified by rapid techniques that do not involve Edman degradation sequencing. These approaches entail the matching of amino acid compositions or tryptic peptide masses of proteins against databases, often in conjunction with estimated protein molecular weight and isoelectric point. As genome sequencing projects progress, proteins from poorly molecularly defined organisms will increasingly be identified by cross-species comparison to proteins from well-defined organisms. To investigate the application of rapid techniques for cross-species protein identification, a total of 65 theoretical cross-species comparisons involving 21 proteins (nine human and 12 E. coli) were undertaken. The degree of conservation of amino acid composition, tryptic peptides, protein isoelectric point and mass was established. Protein amino acid composition was well conserved across species boundaries, whilst tryptic peptides were poorly conserved. The molecular weight of proteins was generally well conserved, but protein isoelectric point was not. These results suggest that cross-species protein identification by rapid techniques will be done best by protein amino acid composition and protein molecular weight.

Citations

Jul 26, 2006·Parasitology·F PontonD G Biron
Aug 10, 2005·The Journal of Biological Chemistry·Ching-Hui YangZissis C Chroneos
Feb 19, 2004·The Journal of Experimental Medicine·Gerard L BannenbergCharles Serhan
Sep 30, 2005·Proceedings. Biological Sciences·D G BironF Thomas
Jun 15, 2011·BMC Microbiology·Pilar Martinez-MoyaVíctor Cifuentes
Jul 20, 2011·Parasites & Vectors·Sirilaksana PatramoolDorothée Missé
May 9, 2008·PLoS Computational Biology·Leo McHugh, Jonathan W Arthur
Aug 29, 2012·PLoS Computational Biology·Victor SeguritanAnca M Segall
Dec 22, 2004·The Journal of Physiology·David R M GrahamJennifer E Van Eyk
Mar 4, 2008·Physiologia Plantarum·Sebastien Christian CarpentierBart Panis
Jun 20, 2012·Mass Spectrometry Reviews·Vincenzo CunsoloSalvatore Foti
Mar 17, 2016·Parasites & Vectors·Martin Bienvenu SomdaBruno Bucheton
Nov 3, 2009·Proteomics·Ladawan WasinpiyamongkolDorothée Missé
Apr 13, 2005·Bio Systems·Malgorzata DudkiewiczStanislaw Cebrat
Oct 27, 2004·Molecular and Biochemical Parasitology·Rachel S CurwenR Alan Wilson
Feb 14, 2006·Protein Expression and Purification·Markus S BirringerGerd Folkers
Aug 17, 2005·International Journal for Parasitology·D G BironY Michalakis
Nov 23, 2006·Current Genetics·Jasmine GrinyerHelena Nevalainen
Apr 15, 2004·Phytochemistry·Raffaella PierleoniVilberto Stocchi
Nov 7, 2003·Genes, Chromosomes & Cancer·Orlev Levy-NissenbaumIsaac P Witz
May 18, 2005·FEBS Letters·Carlo CaporaleCarla Caruso
Oct 30, 2007·Marine Biotechnology·Kuo-Hsun ChiuHin-Kiu Mok
Apr 3, 2008·Mass Spectrometry Reviews·Sebastien Christian CarpentierBart Devreese
Oct 31, 2009·Plant Molecular Biology·Dongxiao LiLiang Chen
Apr 29, 2000·Electrophoresis·P Cash
Aug 3, 2005·Proteomics·Christine R FaulknerRobyn L Overall
Aug 15, 2001·The Journal of Clinical Endocrinology and Metabolism·W J WaltonG R Bousfield
Jul 16, 2013·Biochemistry. Biokhimii︠a︡·D V VasinaO V Koroleva

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.