Cross-species proteomics in analysis of mammalian sperm proteins

Journal of Proteomics
Helen L BayramDean E Hammond

Abstract

Many proteomics studies are conducted in model organisms for which fully annotated, detailed, high quality proteomes are available. By contrast, many studies in ecology and evolution are conducted in species which lack high quality proteome data, limiting the perceived value of a proteomic approach for protein discovery and quantification. This is particularly true of rapidly evolving proteins in the reproductive system, such as those that have an immune function or are under sexual selection, and can compromise the potential for cross-species proteomics to yield confident identification. In this investigation we analysed the sperm proteome, from a range of ungulates and rodents, and explored the potential of routine proteomic workflows to yield characterisation and quantification in non-model organisms. We report that database searching is robust to cross-species matching for a mammalian core sperm proteome, comprising 623 proteins that were common to most of the 19 species studied here, suggesting that these proteins are likely to be present and identifiable across many mammalian sperm. Further, label-free quantification reveals a consistent pattern of expression level. Functional analysis of this core proteome suggests consi...Continue Reading

References

Dec 3, 1999·Journal of Computational Biology : a Journal of Computational Molecular Cell Biology·V DancíkP A Pevzner
Sep 30, 2000·European Journal of Biochemistry·G TedeschiS Ronchi
Feb 28, 2001·Proceedings of the National Academy of Sciences of the United States of America·W J SwansonC F Aquadro
Feb 12, 2002·Nature Reviews. Genetics·Willie J Swanson, Victor D Vacquier
Feb 28, 2003·Biology of Reproduction·Paula R BrownEdward M Eddy
Oct 15, 2003·Rapid Communications in Mass Spectrometry : RCM·Bin MaGilles Lajoie
Jun 1, 2005·Gene·Bianca HaaseTosso Leeb
Jan 3, 2006·Reproduction : the Official Journal of the Society for the Study of Fertility·Nathaniel L ClarkWillie J Swanson
Jun 16, 2006·Molecular Biology and Evolution·Leslie M Turner, Hopi E Hoekstra
Nov 14, 2006·Nature Genetics·Steve DorusTimothy L Karr
Oct 4, 2007·Human Molecular Genetics·Timothy L Karr
Jul 24, 2008·The International Journal of Developmental Biology·Holger Herlyn, Hans Zischler
Oct 27, 2009·Journal of Reproductive Immunology·Shawn Zimmerman, Peter Sutovsky
Nov 26, 2009·Expert Review of Proteomics·Mark A Baker, R John Aitken
Jan 19, 2010·Molecular Biology and Evolution·Steve DorusTimothy L Karr
Apr 9, 2010·Journal of Cell Science·Yoshitaka FujiharaMasaru Okabe
Sep 14, 2010·Journal of Proteomics·Elizabeth R WasbroughTimothy L Karr
Dec 2, 2010·Human Fertility : Journal of the British Fertility Society·Rafael OlivaJosep Lluís Ballescà
Jan 25, 2011·Journal of Proteome Research·Jürgen CoxMatthias Mann
Mar 4, 2011·Reproduction, Fertility, and Development·Georg J Arnold, T Frohlich
Jun 8, 2011·Nucleic Acids Research·Jüri ReimandJaak Vilo
Mar 30, 2012·Omics : a Journal of Integrative Biology·Guangchuang YuQing-Yu He
Jul 14, 2012·Systems Biology in Reproductive Medicine·Steve DorusTimothy L Karr
Nov 20, 2012·Molecular & Cellular Proteomics : MCP·Alexandra AmaralRafael Oliva
Dec 4, 2012·Nucleic Acids Research·Juan Antonio VizcaínoHenning Hermjakob
Jul 3, 2013·Molecular & Cellular Proteomics : MCP·Sheri SkergetTimothy L Karr
Oct 2, 2013·Human Reproduction Update·Alexandra AmaralRafael Oliva
Nov 21, 2013·Nucleic Acids Research·UNKNOWN UniProt Consortium
Jun 18, 2014·The Journal of Cell Biology·Matteo A AvellaJurrien Dean
Mar 4, 2015·Insect Biochemistry and Molecular Biology·Emma WhittingtonSteve Dorus
Apr 22, 2015·Nucleic Acids Research·Damian SmedleyArek Kasprzyk
Jun 16, 2015·Journal of Proteomics·Damien B Wilburn, Willie J Swanson

❮ Previous
Next ❯

Citations

Sep 17, 2016·Journal of Proteome Research·Taylor PiniSimon P de Graaf
Mar 24, 2017·Molecular Biology and Evolution·Alberto VicensSteve Dorus
Feb 4, 2016·Molecular & Cellular Proteomics : MCP·Dean E HammondRobert J Beynon
Aug 18, 2017·Reproduction in Domestic Animals = Zuchthygiene·C Ortega-FerrusolaP Martín-Muñoz
Apr 9, 2020·International Journal of Molecular Sciences·Poshmaal DharSarah Shigdar
Feb 21, 2021·Scientific Reports·Rebecca LazenskyNancy D Denslow
Feb 13, 2019·Theriogenology·Lucía Casares-CrespoMaría P Viudes-de-Castro

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.