Cross-talk among AFAP1-AS1, ACVR1 and microRNA-384 regulates the stemness of pancreatic cancer cells and tumorigenicity in nude mice

Journal of Experimental & Clinical Cancer Research : CR
Xu-Bo WuXi-Qiang Liu

Abstract

Pancreatic cancer (PC) represents one of the most aggressive forms of cancer. The role of long non-coding RNAs (lncRNAs) has been highlighted in various malignancies including PC. The aim of the present study was to investigate the effects associated with actin filament-associated protein 1 antisense RNA 1 (AFAP1-AS1) on the progression of PC and the underlying mechanism. Microarray-based gene expression profiling of PC was performed to identify PC-related lncRNAs, after which the expression of AFAP1-AS1 and cancer stem cell (CSC) markers in PC tissues and cells were determined accordingly. The potential microRNA-384 (miR-384) capable of binding to AFAP1-AS1, in addition to its ability to regulate activin receptor A type I (ACVR1) were analyzed. In order to investigate the effect of the AFAP1-AS1/miR-384/ACVR1 axis on self-renewal ability, tumorigenicity, invasion, migration and stemness of PC cells, shRNA-AFAP1-AS1, miR-384 mimic and inhibitor were cloned into cells. High expression of AFAP1-AS1 and ACVR1 with low expression of miR-384 were detected in PC tissues. ACVR1 was determined to be down-regulated when miR-384 was overexpressed, while the inhibition of AFAP1-AS1 decreased its ability to binding competitively to miR-384...Continue Reading

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Citations

Aug 11, 2020·Journal of Experimental & Clinical Cancer Research : CR·Qing HanZhiwang Song
Jul 3, 2021·International Journal of Molecular Sciences·Jorge Melendez-Zajgla, Vilma Maldonado
Nov 12, 2021·Frontiers in Oncology·Guangbing XiongRenyi Qin

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Datasets Mentioned

BETA
GSE32676
GSE22780

Methods Mentioned

BETA
PCR
electrophoresis
transfection
Pull-Down
immunoprecipitation
RIP
Assay
flow cytometry
chip
pull down

Software Mentioned

RNA22
ImageJ
SPSS

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