Cross-talk between Arg methylation and Ser phosphorylation modulates apoptosis signal-regulating kinase 1 activation in endothelial cells

Molecular Biology of the Cell
Ming ChenWei Shu

Abstract

We describe a novel functional interaction between ASK1 and PRMT5. We show that PRMT5 interacts with and methylates ASK1 at arginine residue 89 and thereby negatively regulates its activity by promoting the interaction between ASK1 and Akt and thus phosphorylating ASK1 at serine residue 83. Furthermore, the association between ASK1 and Akt is enhanced by VEGF stimulation, and PRMT5 is required for this association. Moreover, PRMT5-mediated ASK1 methylation impaired the H2O2-induced activity of ASK1, and this inhibitory effect of PRMT5 was abolished by replacement of arginine 89 with Trp or depletion of PRMT5 expression by RNA interference. Together the results demonstrate cross-talk between arginine methylation and serine phosphorylation in ASK1.

References

Jan 6, 1999·Biochemical and Biophysical Research Communications·X S WangZ Yao
Jan 12, 2001·Molecular and Cellular Biology·A H KimM V Chao
Mar 26, 2003·Cell Structure and Function·Kohsuke TakedaHidenori Ichijo
Jun 29, 2004·Proceedings of the National Academy of Sciences of the United States of America·Hiroaki DaitokuAkiyoshi Fukamizu
Dec 14, 2004·Cell Death and Differentiation·H KadowakiH Ichijo
Mar 7, 2006·Microbes and Infection·Teruyuki HayakawaHidenori Ichijo
May 29, 2007·Methods : a Companion to Methods in Enzymology·Ginny I Chen, Anne-Claude Gingras
Oct 17, 2007·Structure·Gabor BunkocziStefan Knapp
Oct 28, 2008·Molecular Cell·Kazuyuki YamagataAkiyoshi Fukamizu
Nov 12, 2009·Proceedings of the National Academy of Sciences of the United States of America·Daxing XieJer-Tsong Hsieh
Nov 19, 2009·The Journal of Biological Chemistry·Hyun-A SeongHyunjung Ha
Jun 17, 2010·Biochimica Et Biophysica Acta·Stephen J HardingMartin Dickens
Mar 30, 2011·Proceedings of the National Academy of Sciences of the United States of America·Jun-ichi SakamakiAkiyoshi Fukamizu
Jun 21, 2011·Eukaryotic Cell·John C Fisk, Laurie K Read
Nov 18, 2011·Hypertension Research : Official Journal of the Japanese Society of Hypertension·Hisato NakoShokei Kim-Mitsuyama
Nov 19, 2011·Cell Death and Differentiation·J-H ChoE-J Choi
Jun 2, 2012·International Journal of Cell Biology·Mayumi SogaHidenori Ichijo
Jul 12, 2014·The Journal of Biological Chemistry·Ming ChenJianxin Sun

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Citations

Oct 23, 2016·Biochimica Et Biophysica Acta. General Subjects·Chihiro SakauchiKazuki Hattori
Jun 6, 2018·Expert Review of Proteomics·Erica Gianazza, Cristina Banfi
Jan 31, 2019·Molecular Biology of the Cell·Nasim HaghandishJocelyn Côté
Jan 8, 2019·Oncotarget·Harshita ShaileshSaïd Sif
Feb 1, 2019·Signal Transduction and Targeted Therapy·Jihao XuChing-Hsien Chen
Jan 7, 2021·Amino Acids·Sabrina F SamuelPedro Beltran-Alvarez
Feb 12, 2020·Genes & Diseases·Fen Zhu, Lixin Rui
Jan 1, 2021·Epigenetics : Official Journal of the DNA Methylation Society·Melody D FultonY George Zheng
May 20, 2021·Experimental & Molecular Medicine·Jee Won HwangYong Kee Kim
May 7, 2021·Endocrine Reviews·Lucie MalbeteauMuriel Le Romancer

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Methods Mentioned

BETA
transfection
coimmunoprecipitation
immunoprecipitation
PCR

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