Cross-talk between protein kinase C-alpha (PKC-alpha) and -delta (PKC-delta): PKC-alpha elevates the PKC-delta protein level, altering its mRNA transcription and degradation

Biochemistry
L Y RomanovaJ F Mushinski

Abstract

Studies utilizing the overexpression of individual isoforms indicated that both PKC-alpha and -delta promote a number of biological effects, including inhibition of DNA synthesis associated with rearrangements of the actin cytoskeleton in the murine B-cell lymphoma (Baf3), differentiation of the murine promyelocyte line 32D, and activation of MAP kinase in CHO fibroblasts. We postulated that these results reflect some form of cross-regulation between PKC-alpha and -delta rather than their functional redundancy. In this report, we show that overexpression of PKC-alpha in Baf3 and 32D leads to an elevation of the endogenous PKC-delta mRNA and protein levels. The elevated steady-state PKC-delta mRNA level results from a combination of increased PKC-delta transcription and mRNA stability. Upregulation of PKC-delta mRNA by PKC-alpha occurs even after a selective depletion of the PKC-delta protein. In addition, phorbol ester-induced elevation of PKC-delta mRNA and protein levels can be prevented by the PKC inhibitor GF109203X, an indication of the requirement for PKC kinase activity. Inhibition of new protein synthesis by cycloheximide showed that upregulation of PKC-delta mRNA, as opposed to delayed downregulation of the PKC-delta p...Continue Reading

Citations

Dec 2, 2005·Breast Cancer Research and Treatment·Emanuela Maioli, Fortino Vittoria
Jun 16, 2000·Molecular and Cellular Endocrinology·C A PetersM Hunzicker-Dunn
Mar 20, 2010·The Journal of Pharmacology and Experimental Therapeutics·Shanshan LiGuofeng You
Mar 11, 2003·American Journal of Respiratory Cell and Molecular Biology·Arti ShuklaBrooke T Mossman
Apr 15, 2010·Journal of Cell Science·Larisa Y RomanovaJ Frederic Mushinski
Aug 17, 2005·The European Journal of Neuroscience·Yih-Shou HsiehDong-Yih Kuo
Nov 22, 2005·Biochemical and Biophysical Research Communications·Yi-Hsien HsiehJer-Yuh Liu
Dec 9, 2009·Trends in Pharmacological Sciences·Olga Konopatskaya, Alastair W Poole
May 13, 2003·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Fabrice DupratJohn Isaac
Mar 1, 2015·Molecular Pharmacology·Mahlet B Abera, Marcelo G Kazanietz
Jan 15, 2000·The Journal of Biological Chemistry·T Efimova, R L Eckert
Aug 23, 2006·Molecular and Cellular Biochemistry·Gregor SimonisRuth H Strasser
Aug 25, 2000·American Journal of Physiology. Lung Cellular and Molecular Physiology·E C DempseyR O Messing
Dec 31, 2002·American Journal of Physiology. Heart and Circulatory Physiology·Cassana M LittlerEdward C Dempsey
Mar 27, 1999·European Journal of Biochemistry·M Gschwendt
Apr 3, 1999·International Journal of Cancer. Journal International Du Cancer·A Wicki, V Niggli
Jan 18, 2019·Clinical Epigenetics·Chao HeYongqiang Hao
Oct 8, 1999·Biochemical and Biophysical Research Communications·S OshevskiM Castagna
Nov 18, 2006·Biochemical and Biophysical Research Communications·Miriam Horovitz-Fried, Sanford R Sampson
Aug 30, 2008·Molecular and Cellular Neurosciences·Moustapha Alfa CisséBruno Vincent

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