Cross-talk between signaling pathways in murine embryonic palate cells: effect of TGF beta and cAMP on EGF-induced DNA synthesis

In Vitro Cellular & Developmental Biology. Animal
W M WestonRobert M Greene

Abstract

Signaling pathways utilized by EGF, cAMP, and TGF beta have been demonstrated to play critical roles in normal palate development. Stimulation of these pathways has been shown in palate cells and numerous other systems to affect cell growth. Because proper regulation of cell growth is critical to palate development, we speculate that fine regulation of palatal cell growth may be accomplished through crosstalk between these signaling pathways. We therefore set out to determine the effects of cAMP and TGF beta on EGF-induced cell proliferation in murine embryonic palate cells. We found that both TGF beta and cAMP inhibited the proliferative response of cells to treatment with EGF, whereas H89, a serine/ threonine protein kinase inhibitor with selectivity towards cAMP-dependent protein kinase, increased the cells' proliferative response to EGF. Genestein, a selective inhibitor of tyrosine kinases, at high doses abrogated the cells' proliferative response to EGF, confirming that EGF's ability to induce cell proliferation is critically dependent upon tyrosine kinase activity. Lower doses of genestein, however, actually enhanced cellular response to EGF. The data suggest that both the TGF beta- and cAMP-mediated signaling pathways ma...Continue Reading

References

May 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R M Greene, R M Pratt
Dec 1, 1978·Journal of Cellular Physiology·D S Salomon, R M Pratt
Apr 1, 1992·Differentiation; Research in Biological Diversity·A L Gehris, R M Greene
Mar 29, 1991·Biochemical and Biophysical Research Communications·I M KramerW Kruijer
Jan 1, 1989·Critical Reviews in Toxicology·R M Greene
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Jan 1, 1986·Journal of Cellular Physiology·M M PisanoR M Greene
Jan 1, 1984·Current Topics in Developmental Biology·R M PrattR I Grove
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Nov 12, 1993·Science·S J Cook, F McCormick
Oct 1, 1994·In Vitro Cellular & Developmental Biology. Animal·A L GehrisR M Greene

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Citations

Jun 8, 2013·ILAR Journal·Ratnam S SeelanRobert M Greene
Jul 26, 2003·In Vitro Cellular & Developmental Biology. Animal·Vasker BhattacherjeeM Michele Pisano
Jun 11, 2008·Journal of Proteomics·Roman A ZubarevAlexander Kel
Dec 4, 2004·Birth Defects Research. Part A, Clinical and Molecular Teratology·Partha MukhopadhyayM Michele Pisano
Jul 25, 2007·Biochemical and Biophysical Research Communications·Ran Ju KimPyeung-Hyeun Kim
Jun 22, 2000·Teratology·R M Greene, M M Pisano
Jun 17, 2005·Human & Experimental Toxicology·Chada S Reddy
Dec 21, 2006·Birth Defects Research. Part A, Clinical and Molecular Teratology·Saurabh SinghRobert M Greene
Jul 1, 2010·Birth Defects Research. Part A, Clinical and Molecular Teratology·Partha MukhopadhyayRobert M Greene
Jun 15, 2010·Birth Defects Research. Part C, Embryo Today : Reviews·Robert M Greene, M Michele Pisano

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