Cross talk of tumor necrosis factor-alpha and epidermal growth factor in human microvascular endothelial cells

Experimental Cell Research
H IzumiM Kuwano

Abstract

Our previous studies imply that tumor necrosis factor-alpha (TNF-alpha) and epidermal growth factor (EGF) might share a common signal transduction pathway in human omental microvascular endothelial (HOME) cells. Exposure of cultured HOME cells to TNF-alpha for 10 min enhanced EGF receptor phosphorylation at a rate comparable to EGF. Apparent phosphorylation of tyrosine residues was observed in addition to serine/threonine of the EGF receptor by EGF, but only a slightly if any tyrosine phosphorylation by TNF-alpha. In vitro kinase activity of EGF receptor was also enhanced by TNF-alpha as well as by EGF. Furthermore, expression of the c-fos gene was enhanced in response to either EGF or TNF-alpha. Pretreatment of HOME cells with EGF for 12 h almost completely blocked the induction of the c-fos gene by EGF and partially blocked the c-fos induction by TNF-alpha. TNF-alpha-induced c-fos gene expression appeared to be partly due to its transactivation of EGF receptor. EGF and TNF-alpha could enhance c-fos gene expression when protein kinase C was down-regulated by phorbol ester myristate (PMA). Gel retardation assay with the NF-kappa B consensus sequence showed that NF-kappa B binding activity was dramatically activated by TNF-alpha...Continue Reading

Citations

Mar 22, 2008·Proceedings of the National Academy of Sciences of the United States of America·Pak Kin WongChih-Ming Ho
Jun 8, 1999·Annual Review of Immunology·D WallachM P Boldin
Dec 20, 2005·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Koichi AndoToshio Kuroki
Dec 19, 2020·American Journal of Physiology. Heart and Circulatory Physiology·Shun-Guang WeiRobert B Felder

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