PMID: 8948471Nov 13, 1996Paper

Crosslinking of aminophospholipids in cellular membranes of lens by oxidative stress in vitro

Biochimica Et Biophysica Acta
D K BhuyanK C Bhuyan

Abstract

We have previously demonstrated by TLC an additional phospholipid spot between phosphatidylethanolamine (PE) and phosphatidylserine (PS) in human cataract. This was further identified as a fluorescent Schiff-base conjugate resulting from crosslinking of reactive carbonyl groups of malondialdehyde (MDA) with the primary amino groups of membrane phospholipids. Evidence presented here shows that such an adduct could be formed in rabbit lens subjected to oxidative stress in vitro. TLC analysis of a lipid extract of a crude membrane fraction obtained from the lens homogenate incubated with 1 mM H2O2, tert-butyl hydroperoxide (TBHP) or MDA for 1-6 h at 25 degrees C, showed that the oxidants and MDA produced time-dependent crosslinking of aminophospholipids. Under identical conditions of incubation with TBHP or MDA, development of the Schiff-base lipid fluorochrome in lens with peak emission at 470 nm when excited at 360 nm also showed a time-dependent increase. The PE.MDA.PS produced in cellular membranes of the lenses cultured for 3 h in Krebs-Ringer medium was 151 nmol/mumol PE, and addition of 1 mM H2O2, TBHP or MDA, increased it to 881, 610 and 375 nmol/mumol PE, respectively. Adduct was also formed when authentic samples of PE a...Continue Reading

References

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Citations

Nov 19, 2004·Der Ophthalmologe : Zeitschrift der Deutschen Ophthalmologischen Gesellschaft·N SerbecicS C Beutelspacher
Apr 20, 2005·Cell and Tissue Research·Nermin Serbecic, Sven Christoph Beutelspacher
Mar 5, 2004·Chemistry and Physics of Lipids·M Cecilia Yappert, Douglas Borchman
Feb 4, 2010·Molecular Interventions·Alison L Groeger, Bruce A Freeman
Jun 6, 2009·Vojnosanitetski pregled. Military-medical and pharmaceutical review·Bojana KisićAleksandra Stolić
Feb 7, 2013·International Journal of Molecular Sciences·Alba NaudíReinald Pamplona
Feb 13, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Shalene SankhagowitNoah Malmstadt
Jul 24, 2012·Biochimie·Lilu Guo, Sean S Davies
Aug 2, 2003·Organic Letters·Bogdan G Gugiu, Robert G Salomon
Feb 19, 2002·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·Rajeev BuddiDonald J Brown
Sep 6, 2003·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Kuiyi Xing, Marjorie F Lou

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