PMID: 11342214May 9, 2001Paper

Crosslinking of the complementary strands of DNA by UV light: dependence on the oligonucleotide composition of the UV irradiated DNA

Biochimica Et Biophysica Acta
K NejedlýJ Kypr

Abstract

UV light crosslinks the complementary strands of DNA. The interstrand crosslinks may contribute to the biological and pathological effects that UV irradiation is known to bring about. Here alkaline agarose gel electrophoresis was used to assess the crosslinked fraction of 31 selected restriction fragments of six viral and plasmid DNA molecules exposed to UVC light irradiation. As many as 17 independent experiments were performed with the particular DNA fragments to get sufficiently precise data suitable for quantitative analyses. The data were used to determine how the crosslinked fraction depended on the dinucleotide, trinucleotide and tetranucleotide contents of the irradiated DNA fragments. This analysis demonstrated that DNA conformation and/or flexibility, rather than the local double helix thermostability, governed the phenomenon of crosslinking. For example, (GA).(TC) suppressed the crosslink formation in DNA more than any dinucleotide composed of only G and C. In addition, (CTAG).(CTAG) promoted crosslinking much more than any other tetranucleotide, including e.g. (TATA).(TATA), whereas the closely related (CATG).(CATG) belonged among the tetranucleotides that most suppressed the UV light induced crosslinks between the ...Continue Reading

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Citations

Jan 13, 2005·Nucleic Acids Research·Karel NejedlýJaroslav Kypr
Jul 26, 2005·Radiation Research·Zhongli CaiDarel Hunting
Mar 28, 2009·International Journal of Molecular Sciences·Kathryn SchallhornPu Chun Ke
Jun 11, 2015·The Journal of Physical Chemistry. B·Janusz RakŁukasz Golon
Mar 23, 2005·Cancer Research·Phillip G SmiraldoDouglas L Pittman

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