Feb 29, 2020

Crosstalk between heterotrimeric G protein-coupled signaling pathways and WRKY transcription factors modulating plant responses to suboptimal micronutrient conditions

Journal of Experimental Botany
Ting-Ying WuDaisuke Urano

Abstract

Nutrient stresses induce foliar chlorosis and growth defects. Here we propose heterotrimeric G protein as signaling mediators of various nutrient stresses, through meta-analyses of more than 20 transcriptomic data associated with nutrient stresses or G-protein mutations. Systematic comparison of transcriptomic data yielded 104 genes regulated by G protein subunits under common nutrient stresses; 69 genes under Gβ subunit (AGB1) and 35 genes under Gα subunit (GPA1) control. Quantitative RT-PCR experiments validate that several transcription factors and metal transporters changed in expression level under suboptimal iron, zinc and/or copper concentrations, while being mis-regulated in Arabidopsis Gβ-null (agb1) mutant. The agb1 mutant altered metal ion profiles and exhibited severe growth arrest under zinc stress and aberrant root waving under iron and zinc stresses, while Gα-null mutation attenuated leaf chlorosis under iron deficiency both in Arabidopsis and rice. Our transcriptional network analysis inferred computationally that WRKY-family transcription factors mediate the AGB1-dependent nutrient responses. As corroborating evidence of our inference, ectopic expression of WRKY25 or WRKY33 rescued the zinc-stress phenotypes an...Continue Reading

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Mentioned in this Paper

Ectopic Expression
Plant Leaves
Gene Regulatory Networks
Arabidopsis
Zinc transporter
Growth Arrest
Modulated
Gene Ontology
Null Allele
Copper

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